| 生物学的情報 |
The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
|
| 説明 |
AMPK (G1/B1/A1) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 160 kDa and the accession number is P54619-1&Q9Y478-1&Q13131-1. |
| Species |
Human
|
| Expression Host |
Baculovirus Insect Cells
|
| Tag |
His, GST
|
| Accession Number |
P54619-1&Q9Y478-1&Q13131-1
|
| Construction |
The Human PRKAG1 (P54619-1) (Met1-Pro331), constructed the plasmid 1; The Human PRKAB1 (Q9Y478-1) (Met1-Ile270), constructed the plasmid 2; The Human PRKAA1 (Q13131-1) (Met1-Gln559) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purifie.
|
| Protein Purity |
> 85 % as determined by SDS-PAGE
|
| 分子量 |
160 kDa (predicted)
|
| Endotoxin |
< 1.0 EU/μg of the protein as determined by the LAL method.
|
| Formulation |
Supplied as sterile 20 mM Tris, 300 mM NaCl, 10% Glycerol, pH 7.5.
|
| Reconstitution |
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
|
| Stability & Storage |
It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
|
| Shipping |
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
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