| 説明 |
AMPK (G1/B2/A2) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 158 kDa and the accession number is P54619&Q9Y478&P54646. |
| Species |
Human
|
| Expression Host |
Baculovirus Insect Cells
|
| Tag |
His, GST
|
| Accession Number |
P54619&Q9Y478&P54646
|
| Construction |
The Human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; The Human PRKAB2 (O43741) (Met 1-Ile 272), constructed the plasmid 2; The Human PRKAA2 (P54646) (Met 1-Arg 552) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.
|
| Protein Purity |
> 90 % as determined by SDS-PAGE
|
| 分子量 |
158 kDa (predicted)
|
| Endotoxin |
< 1.0 EU/μg of the protein as determined by the LAL method.
|
| Formulation |
Supplied as sterile 50 mM Tris, 200 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.5 mM PMSF, 10% gly, 1 mM GSH, pH 7.4.
|
| Reconstitution |
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
|
| Stability & Storage |
It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
|
| Shipping |
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
|
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