ART4/CD297 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 28.2 kDa and the accession number is EDL10575.1.
説明 | ART4/CD297 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 28.2 kDa and the accession number is EDL10575.1. |
Species | Mouse |
Expression Host | HEK293 Cells |
Tag | C-His |
Accession Number | EDL10575.1 |
別名 | ADP-ribosyltransferase 4 (Dombrock blood group), ARTC4, 4432404K01Rik, DO, DOK1 |
Construction | The mature form of Mouse ART4 (EDL10575.1) (Met1-Lys 263) was expressed, with a C-terminal polyhistidine tag. |
Protein Purity | > 92 % as determined by SDS-PAGE |
分子量 | 28.2 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | ADP-ribosyltransferase 4 (Dombrock blood group), also known as Mono-ADP-ribosyltransferase 4(ART4), Dombrock blood group carrier molecule and CD297, is a protein that contains a mono-ADP-ribosylation (ART) motif. It is a member of the ADP-ribosyltransferase gene family but enzymatic activity has not been demonstrated experimentally. ADP-ribosyltransferase catalyzes the ADP-ribosylation of arginine residues in proteins. Mono-ADP-ribosylation is a posttranslational modification of proteins that is interfered with by a variety of bacterial toxins including cholera, pertussis, and heat-labile enterotoxins of E. coli. ART4 could be detected on HEL cells and erythrocytes by FACS analysis while it was absent from activated monocytes, despite the presence of ART4 mRNA in these cells. ART is also known as the carrier of the Dombrock blood group alloantigens (Do) which is glycosylphosphatidylinosotol-anchored to the erythrocyte membrane. |
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Please read the User Guide of Recombinant Proteins for more specific information.
ART4/CD297 Protein, Mouse, Recombinant (His) ADP-ribosyltransferase 4 (Dombrock blood group) ARTC4 DOK-1 DOK 1 Dombrock blood group 4432404K01Rik ARTC 4 ARTC-4 DO ADP-ribosyltransferase 4 DOK1 recombinant recombinant-proteins proteins protein