B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R. BAFF/TNFSF13B Trimer Protein, Human, Recombinant (His & Flag) is expressed in HEK293 mammalian cells with N-His-Flag tag. The predicted molecular weight is 52.4 kDa and the accession number is Q9Y275-1.
| 生物学的情報 |
1. Immobilized Human BAFF Trimer, His Tag at 1μg/ml (100μl/well). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 37.8ng/ml determined by ELISA.
2. Human BAFF Trimer, His Tag captured on CM5 Chip via Anti-His Antibody can bind Mouse BCMA, hFc Tag with an affinity constant of 6.59 nM as determined in SPR assay.
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| 説明 |
B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R. BAFF/TNFSF13B Trimer Protein, Human, Recombinant (His & Flag) is expressed in HEK293 mammalian cells with N-His-Flag tag. The predicted molecular weight is 52.4 kDa and the accession number is Q9Y275-1. |
| Species |
Human
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| Expression Host |
HEK293 Cells
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| Tag |
N-His-Flag
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| Accession Number |
Q9Y275-1
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| 別名 |
BLyS, TALL-1, TNFSF13B, DTL, TNFSF20, TALL-1δ BAFF, TALL-1delta BAFF, TALL1δ4 BAFF, ZTNF4, TALL1, THANK, BAFF, TALL1Delta4 BAFF, CD257
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| Construction |
Thr141-Leu285
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| Protein Purity |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
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| 分子量 |
52.4 kDa (predicted). Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result.
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| Endotoxin |
< 1 EU/μg by the LAL method.
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| Formulation |
Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
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| Reconstitution |
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
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| Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
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| Shipping |
In general, Lyophilized powders are shipping with blue ice.
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| Research Background |
B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.
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参考文献
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