Complement C5 Protein, Human, Recombinant (His & FLAG) is expressed in HEK293 mammalian cells with His and Flag tag. The predicted molecular weight is 188.8 kDa and the accession number is P01031.
パッケージサイズ | 在庫状況 | 単価(税別) |
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100 μg | 在庫あり | ¥ 75,500 |
200 μg | 約5 days | ¥ 134,000 |
500 μg | 約5 days | ¥ 282,500 |
生物学的情報 |
1.Loaded Eculizumab on ProA Biosensor, can bind Recombinant Human C5 Protein, His & FLAG Tag with an affinity constant of 1.85nM as determined in BLI assay (Routinely tested).
2. Immobilized Anti-C5 Antibody at 2 μg/mL (100 μL/well) can bind Recombinant Human C5 / Complement 5 Protein (Flag & His Tag), the EC50 is 25-75 ng/mL.
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説明 | Complement C5 Protein, Human, Recombinant (His & FLAG) is expressed in HEK293 mammalian cells with His and Flag tag. The predicted molecular weight is 188.8 kDa and the accession number is P01031. |
Species | Human |
Expression Host | HEK293 Cells |
Tag | His, Flag |
Accession Number | P01031 |
別名 | C5b, ECLZB, C5a, CPAMD4, C5D, complement component 5, Complement C5 |
Construction | The Human C5 (NP_001726.2) (Gln19-Cys1676) was expressed with a polyhistidine tag at the C-terminus and a FLAG tag at the N-terminus. |
Protein Purity |
> 95 as determined by SDS-PAGE.
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分子量 | 188.8 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | C5a is a protein fragment released from complement component C5. This 74 amino acid peptide in humans is generated by the cleavage of C5a convertase on the C5 α-chain during the classical, alternative, and lectin pathways of complement activation. The structure of C5a includes a core region consisting of four, anti-parallel alpha-helices held together by three disulfide linkages and a structured C-terminal tail, and C5a is rapidly metabolised by carboxypeptidase B to a 73 amino acid low activity form, C5a des-Arg. C5a is an extremely potent proinflammatory mediator, as well as a potent chemotactic factor for neutrophils and other leukocytes. It causes histamine release, increases in vascular permeability, induces several cytokines production from leukocytes, enhances neutrophil-endothelial cell adhesion, and augments the humoral and cell-mediated immune response. C5a is quickly metabolised by carboxypeptidases, forming the less potent C5adesArg. Acting via a classical G protein-coupled receptor, CD88, C5a and C5adesArg exert a number of effects essential to the innate immune response, while their actions at the more recently discovered non-G protein-coupled receptor, C5L2 (or GPR77), remain unclear. The widespread expression of C5a receptors throughout the body allows C5a to elicit a broad range of effects. Thus, C5a has been found to be a significant pathogenic driver in a number of immuno-inflammatory diseases, making C5a inhibition an attractive therapeutic strategy. C5a is a strong chemoattractant and is involved in the recruitment of inflammatory cells such as neutrophils, eosinophils, monocytes, and T lymphocytes, in activation of phagocytic cells and release of granule-based enzymes and generation of oxidants, all of which may contribute to innate immune functions or tissue damage. Accordingly, the anaphylatoxin C5a is implicated in a variety of diseases such as rheumatoid arthritis, systemic lupus erythematosus, reperfusion injury, Alzheimer's disease, and sepsis. |
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Please read the User Guide of Recombinant Proteins for more specific information.
Complement C5 Protein, Human, Recombinant (His & FLAG) C5b ECLZB C5a CPAMD4 C5D complement component 5 CPAMD-4 CPAMD 4 Complement C5 recombinant recombinant-proteins proteins protein