Carbonic Anhydrase 10 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 27.2 kDa and the accession number is A0A384MTY8.
説明 | Carbonic Anhydrase 10 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 27.2 kDa and the accession number is A0A384MTY8. |
Species | Human |
Expression Host | HEK293 Cells |
Tag | Tag Free |
Accession Number | A0A384MTY8 |
別名 | carbonic anhydrase X, CARPX, CA-RPX, HUCEP-15, CA10 |
Construction | The mature form of Human CARPX (NP_001076002.1) (Met 1-Asn 300) with five amino acids (DDDDK) at the C-terminus was expressed and purified. |
Protein Purity | > 95 % as determined by SDS-PAGE |
分子量 | 27.2 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing 100 mM Glycine, 10 mM NaCl, 50 mM Tris, pH 7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | Carbonic anhydrase X, also called carbonic anhydrase - related protein X (CARPX) and CA1, belongs to the CA family of zinc metalloenzymes which catalyze the reversible hydration of carbon dioxide in various biological processes such as respiration, renal tubular acidification and bone resorption. The secreted protein CARPX without CA activity (hydration of CO2) is identified as an acatalytic member of the alpha-carbonic anhydrase subgroup. CARP X expression is detected in the adult total brain and almost all parts of the central nervous system, but not in the fetal brain. Accordingly, CARP X is suggested to play a role in the development of central nervous system, especially the brain. The same CARP X protein are encoded by multiple transcript variants of this gene. |
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Please read the User Guide of Recombinant Proteins for more specific information.
Carbonic Anhydrase 10 Protein, Human, Recombinant HUCEP15 CA 10 carbonic anhydrase X CARPX HUCEP 15 CA-RPX HUCEP-15 CA10 CA-10 recombinant recombinant-proteins proteins protein