Carbonic Anhydrase 10 Protein, Mouse, Recombinant (His)

Carbonic Anhydrase 10 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 33.1 kDa and the accession number is P61215.

Carbonic Anhydrase 10 Protein, Mouse, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 161,000

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説明	Carbonic Anhydrase 10 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 33.1 kDa and the accession number is P61215.

Species	Mouse
Expression Host	HEK293 Cells
Tag	C-His
Accession Number	P61215
別名	carbonic anhydrase X, BB085816, RP23-448I9.1, Car10, 2700029L05Rik, Ca10
Construction	The Mouse Car10 (P61215) (Met 1-Asn 300) was expressed, fused with a C-terminal polyhistidine tag.
Protein Purity	> 97 % as determined by SDS-PAGE
分子量	33.1 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 25 mM Tris, 150 mM NaCl, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Carbonic anhydrase X, also called carbonic anhydrase - related protein X (CARPX) and CA1, belongs to the CA family of zinc metalloenzymes which catalyze the reversible hydration of carbon dioxide in various biological processes such as respiration, renal tubular acidification and bone resorption. The secreted protein CARPX without CA activity (hydration of CO2) is identified as an acatalytic member of the alpha-carbonic anhydrase subgroup. CARP X expression is detected in the adult total brain and almost all parts of the central nervous system, but not in the fetal brain. Accordingly, CARP X is suggested to play a role in the development of central nervous system, especially the brain. The same CARP X protein are encoded by multiple transcript variants of this gene.

参考文献

1. Okamoto, N. et al., 2001, Biochim. Biophys. Acta 1518:311-316. 2. Taniuchi, K. et al., Neuroscience 112: 93-99. 3. Supuran, C.T. et al., 2003, Med. Res. Rev. 23: 146-189.

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Carbonic Anhydrase 10 Protein, Mouse, Recombinant (His)

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