Carbonic Anhydrase 12 Protein, Human, Recombinant (His)

Carbonic Anhydrase 12 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 31.6 kDa and the accession number is O43570-1.

Carbonic Anhydrase 12 Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
50 μg	在庫あり	￥ 67,500
100 μg	約5 days	￥ 113,000
200 μg	約5 days	￥ 189,000
500 μg	約5 days	￥ 374,000

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生物学的情報	Measured by its esterase activity. The specific activity is >40 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 2.5 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
説明	Carbonic Anhydrase 12 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 31.6 kDa and the accession number is O43570-1.

Species	Human
Expression Host	HEK293 Cells
Tag	C-His
Accession Number	O43570-1
別名	HsT18816, FLJ20151, carbonic anhydrase XII, CA12, CAXII
Construction	extracellular domain of Human CA12 (NP_001209.1) (Met 1-Gln 291)
Protein Purity	≥ 97 % as determined by SDS-PAGE ≥ 90 % as determined by SEC-HPLC.
分子量	31.6 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA12, also known as Car12 and carbonic anhydrase XII, is a type I membrane enzyme of an N-terminal extracellular catalytic domain, a membrane-spanning α-helix, and a small intracellular C-terminal domain. It is highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis. Overexpression of the CA12 is observed in certain human cancers and is used as a tumor marker. rmCA12 corresponds to the extracellular domain and has both carbonic anhydrase activity and esterase activity.

参考文献

1. Sahin, U. et al., 1996, Proc. Natl. Acad. Sci. U.S.A. 92 (25): 11810–11813. 2. Ivanov, S.V. et al., 1998, Proc. Natl. Acad. Sci. USA 95:12596 - 12601. 3. Strausberg, R.L. et al., 2002, Proc. Natl. Acad. Sci. USA 99:16899 - 16903. 4. Liao, S.Y. et al., 2003, J. Med. Genet. 40:257 - 262. 5. Supuran, C. T. et al., 2008, Curr Pharm Des. 14 (7): 601-602. 6. Elleuche, S. et al., 2009, Curr Genet. 55 (2): 211-222.

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Carbonic Anhydrase 12 Protein, Human, Recombinant (His)

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