B3GAT1 is the key enzyme during the biosynthesis of the carbohydrate epitope HNK-1, which is present on a number of cell adhesion molecules important in neurodevelopment. Saturating concentrations of rVAR2 inhibited downstream integrin signaling, which was mimicked by knockdown of the core chondroitin sulfate synthesis enzymes β-1,3-glucuronyltransferase 1 (B3GAT1) and chondroitin sulfate N-acetylgalactosaminyltransferase 1 (CSGALNACT1).
説明 | B3GAT1 is the key enzyme during the biosynthesis of the carbohydrate epitope HNK-1, which is present on a number of cell adhesion molecules important in neurodevelopment. Saturating concentrations of rVAR2 inhibited downstream integrin signaling, which was mimicked by knockdown of the core chondroitin sulfate synthesis enzymes β-1,3-glucuronyltransferase 1 (B3GAT1) and chondroitin sulfate N-acetylgalactosaminyltransferase 1 (CSGALNACT1). |
Species | Human |
Expression Host | HEK293 Cells |
Tag | N-mFc |
Accession Number | Q9P2W7-1 |
別名 | LEU7, NK1, beta-1,3-glucuronyltransferase 1, HNK1, GLCATP, NK-1, β-1,3-glucuronyltransferase 1, GLCUATP, CD57 |
Construction | The Human B3GAT1 (NP_473366.1) (Thr28-Ile334) was expressed with the Fc region of Mouse IgG1 at the N-terminus. |
Protein Purity | > 90 % as determined by SDS-PAGE. |
分子量 | 61.7 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | B3GAT1 is the key enzyme during the biosynthesis of the carbohydrate epitope HNK-1, which is present on a number of cell adhesion molecules important in neurodevelopment. Saturating concentrations of rVAR2 inhibited downstream integrin signaling, which was mimicked by knockdown of the core chondroitin sulfate synthesis enzymes β-1,3-glucuronyltransferase 1 (B3GAT1) and chondroitin sulfate N-acetylgalactosaminyltransferase 1 (CSGALNACT1). |
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CD57/B3gat1 Protein, Human, Recombinant (mFc) LEU7 HNK 1 CD 57 NK 1 NK1 HNK-1 LEU-7 beta-1,3-glucuronyltransferase 1 CD-57 HNK1 GLCATP NK-1 b-1,3-glucuronyltransferase 1 β-1,3-glucuronyltransferase 1 GLCUATP CD57 LEU 7 recombinant recombinant-proteins proteins protein