DAPK1 Protein, Human, Recombinant (aa 1-363, His & GST)

カタログ番号 TMPY-04417

別名: DAPK, death-associated protein kinase 1, ROCO3

Death-associated protein kinase 1, also known as DAP kinase 1, DAPK1 and DAPK, is a cytoplasm protein which belongs to theprotein kinase superfamily, CAMK Ser / Thr protein kinase family and DAP kinase subfamily. DAPK1 contains tenANK repeats, onedeath domain and oneprotein kinase domain. DAPK1 is a calcium / calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis. DAPK1 gene is a candidate tumor suppressor (TSG) and the abnormal methylation of DAPK1 gene has been found in many carcinomas. DAPK1 over-expression can induce cell apoptosis and inhibit tumor cell metastasis. DAPK1 gene over-expression could suppress PGCl3 cells malignant phenotype, inhibit PGCl3 cells growth, invasive, migration and adhesion ability, upregulate p53 gene and downregulate bcl-2 gene. Loss of activity of death-associated protein kinase 1 ( DAPK1 ) may be an independent factor affecting survival of non-small cell lung cancer patients. DAPK1 promoter methylation might play a significant role in the progression of chronic myeloid leukemia ( CML ).

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DAPK1 Protein, Human, Recombinant (aa 1-363, His & GST)

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 114,500

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生物学的特性に関する説明

Technical Params

Product Properties

参考文献

説明

Species	Human
Expression Host	Baculovirus Insect Cells
Tag	His, GST
Accession Number	P53355-1
別名	DAPK, death-associated protein kinase 1, ROCO3
Construction	The N-terminal segment of Human DAPK1 (P53355-1) (Met 1-Leu 363) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Protein Purity	> 80 % as determined by SDS-PAGE
分子量	69.4 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10% gly.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Research Background	Death-associated protein kinase 1, also known as DAP kinase 1, DAPK1 and DAPK, is a cytoplasm protein which belongs to theprotein kinase superfamily, CAMK Ser / Thr protein kinase family and DAP kinase subfamily. DAPK1 contains tenANK repeats, onedeath domain and oneprotein kinase domain. DAPK1 is a calcium / calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis. DAPK1 gene is a candidate tumor suppressor (TSG) and the abnormal methylation of DAPK1 gene has been found in many carcinomas. DAPK1 over-expression can induce cell apoptosis and inhibit tumor cell metastasis. DAPK1 gene over-expression could suppress PGCl3 cells malignant phenotype, inhibit PGCl3 cells growth, invasive, migration and adhesion ability, upregulate p53 gene and downregulate bcl-2 gene. Loss of activity of death-associated protein kinase 1 ( DAPK1 ) may be an independent factor affecting survival of non-small cell lung cancer patients. DAPK1 promoter methylation might play a significant role in the progression of chronic myeloid leukemia ( CML ).

参考文献

1. Zhang,H.T. et al., 2004, Ai Zheng. 23 (5):497-501. 2. Martoriati,A. et al., 2005, Oncogene. 24 (8):1461-6. 3. Li,Y. et al., 2006, Hum Mol Genet. 15 (17): 2560-8. 4. Martinez-Glez,V. et al., 2007, Neoplasma. 54 (2):123-6. 5. Raval,A. et al., 2007, Cell. 129 (5):879-90. 6. Gade,P. et al., 2009, Int J Cancer. 125 (7):1566-74.

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Keywords

DAPK1 Protein, Human, Recombinant (aa 1-363, His & GST) DAPK death-associated protein kinase 1 ROCO 3 ROCO-3 ROCO3 recombinant recombinant-proteins proteins protein