EGFR Protein, Human, Recombinant (His)

EGFR Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 69.8 kDa and the accession number is P00533-1.

EGFR Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 88,500
200 μg	約5 days	￥ 160,000
500 μg	約5 days	￥ 349,000

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生物学的情報

1.Measured by its binding ability in a functional ELISA. Immobilized Human EGF hFc at 2 μg/ml (100 μl/well) can bind Human EGFR His , the EC50 of Human EGFR His is 100-500 ng/mL. 2. Captured Cetuximab (IgG1) on proA Chip can bind Human EGFR recombinant protein with an affinity constant of 1.07 nM as determined in an SPR assay (Routinely tested). 3. Loaded Cetuximab on ProA Biosensor, can bind Human EGFR protein, His Tag with an affinity constant of 5.81nM as determined in BLI assay (Routinely tested).

説明

EGFR Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 69.8 kDa and the accession number is P00533-1.

Species	Human
Expression Host	HEK293 Cells
Tag	C-His
Accession Number	P00533-1
別名	mENA, NISBD2, ERBB1, HER1, PIG61, ERBB, epidermal growth factor receptor
Construction	extracellular domain (Met 1-Ser 645) of Human EGFR (NP_005219)
Protein Purity	> 95 % as determined by SDS-PAGE. >90% as determined by SEC-HPLC
分子量	69.8 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

参考文献

1. Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25. 2. Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48. 3. Yarden, Y.,et al.(2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.

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EGFR Protein, Human, Recombinant (His)

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