| 説明 |
EGFR Protein, Rhesus macaque, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 69.8 kDa and the accession number is P55245. |
| Species |
Rhesus
|
| Expression Host |
HEK293 Cells
|
| Tag |
C-His
|
| Accession Number |
P55245
|
| 別名 |
HER1, EC 2.7.10, EGFR, NISBD2, ErbB, PIG61, ERBB1, LEGFR, mENA, EC 2.7.10.1
|
| Construction |
Leu25-Ser645
|
| Protein Purity |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
|
| 分子量 |
69.8 kDa (predicted). Due to glycosylation, the protein migrates to 85-100 kDa based on Tris-Bis PAGE result.
|
| Endotoxin |
< 1 EU/μg by the LAL method.
|
| Formulation |
Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
|
| Reconstitution |
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
|
| Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
|
| Shipping |
In general, Lyophilized powders are shipping with blue ice.
|
| Research Background |
The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.
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参考文献
Calculator
Reconstitution Calculator
Recombinant Proteins Dilute Calculator
Specific Activity Calculator
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