The epidermal growth factor receptor (EGFR) is overexpressed in a variety of human epithelial tumors, often as a consequence of gene amplification. Tumors with EGFR gene amplification frequently contain EGFR gene rearrangements, with the most common extracellular domain mutation being EGFRvIII. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand. EGFRVIII Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 41.6 kDa and the accession number is NP_001333870.1.
パッケージサイズ | 在庫状況 | 単価(税別) |
---|---|---|
100 μg | 約5 days | ¥ 187,000 |
500 μg | 約5 days | ¥ 746,000 |
1 mg | 約5 days | ¥ 1,248,500 |
説明 | The epidermal growth factor receptor (EGFR) is overexpressed in a variety of human epithelial tumors, often as a consequence of gene amplification. Tumors with EGFR gene amplification frequently contain EGFR gene rearrangements, with the most common extracellular domain mutation being EGFRvIII. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand. EGFRVIII Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 41.6 kDa and the accession number is NP_001333870.1. |
Species | Human |
Expression Host | HEK293 Cells |
Tag | C-His-Avi |
Accession Number | NP_001333870.1 |
別名 | EC 2.7.10.1, PIG61, NISBD2, HER1, mENA, LEGFR, ErbB, EC 2.7.10, ERBB1, EGFR |
Construction | Leu25-Ser378 |
Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | 41.6 kDa (predicted). Due to glycosylation, the protein migrates to 60-78 kDa based on Tris-Bis PAGE result. |
Endotoxin | < 1 EU/μg by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | The epidermal growth factor receptor (EGFR) is overexpressed in a variety of human epithelial tumors, often as a consequence of gene amplification. Tumors with EGFR gene amplification frequently contain EGFR gene rearrangements, with the most common extracellular domain mutation being EGFRvIII. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand. |
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Please read the User Guide of Recombinant Proteins for more specific information.
EGFRVIII Protein, Human, Recombinant (His & Avi), Biotinylated EC 2.7.10.1 PIG61 NISBD2 HER-1 ERBB 1 HER1 NISBD-2 mENA LEGFR PIG-61 ErbB HER 1 ERBB-1 PIG 61 NISBD 2 EC 2.7.10 ERBB1 EGFR recombinant recombinant-proteins proteins protein