GDF-15 Protein, Canine, Recombinant (His)

Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15. GDF-15 Protein, Canine, Recombinant (His) is expressed in E. coli expression system with N-His tag. The predicted molecular weight is 13.12 kDa and the accession number is A0A8C0TNP6.

GDF-15 Protein, Canine, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 160,500
500 μg	約5 days	￥ 643,000
1 mg	約5 days	￥ 1,067,500

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説明

Species	Canine
Expression Host	E. coli
Tag	N-His
Accession Number	A0A8C0TNP6
別名	GDF15, MIC1, MIC-1, PLAB, GDF-15, PDF, PTGFB, PTGF-beta, RG-1, Placental TGF-β, PTGF-β, PTGFBPTGF-beta, NAG-1, Placental TGF-beta, PTGFBPTGF-β
Construction	Ala197-Val307
Protein Purity	> 95% as determined by Tris-Bis PAGE
分子量	13.12 kDa (predicted). The protein migrates to 15-20 kDa under reduced (R) condition and 23-26 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM HAc (pH 2.9). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in 50mM HAc (pH 2.9). The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15.

参考文献

1. Baek SJ, Eling T. Growth differentiation factor 15 (GDF15): A survival protein with therapeutic potential in metabolic diseases. Pharmacol Ther. 2019 Jun;198:46-58. doi: 10.1016/j.pharmthera.2019.02.008. Epub 2019 Feb 18. PMID: 30790643; PMCID: PMC7196666.

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GDF-15 Protein, Canine, Recombinant (His)

参考文献

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