GDF-15 Protein, Cynomolgus, Recombinant (His)

Growth and differentiation factor 15 (GDF15) is an inflammation-associated hormone with poorly defined biology. Here, we investigated the role of GDF15 in bacterial and viral infections. Inflammation induced GDF15, and that GDF15 was necessary for surviving both bacterial and viral infections, as well as sepsis. The protective effects of GDF15 were largely independent of pathogen control or the magnitude of inflammatory response, suggesting a role in disease tolerance. GDF-15 Protein, Cynomolgus, Recombinant (His) is expressed in E. coli expression system with N-His tag. The predicted molecular weight is 14.15 kDa and the accession number is G7PWZ3.

GDF-15 Protein, Cynomolgus, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 112,000
500 μg	約5 days	￥ 447,500
1 mg	約5 days	￥ 746,000

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説明

Species	Cynomolgus
Expression Host	E. coli
Tag	N-His
Accession Number	G7PWZ3
別名	PTGFBPTGF-beta, PTGFBPTGF-β, MIC-1, NAG-1, MIC1, PTGF-beta, PTGF-β, Placental TGF-β, PLAB, Placental TGF-beta, PTGFB, RG-1, PDF, GDF-15, GDF15
Construction	Arg193-Val308
Protein Purity	> 95% as determined by Tris-Bis PAGE
分子量	14.15 kDa (predicted). The protein migrates to 15-18 kDa based on Tris-Bis PAGE result.

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM HAc (pH 2.9). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in 50mM HAc (pH 2.9). The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Growth and differentiation factor 15 (GDF15) is an inflammation-associated hormone with poorly defined biology. Here, we investigated the role of GDF15 in bacterial and viral infections. Inflammation induced GDF15, and that GDF15 was necessary for surviving both bacterial and viral infections, as well as sepsis. The protective effects of GDF15 were largely independent of pathogen control or the magnitude of inflammatory response, suggesting a role in disease tolerance.

参考文献

1. Luan HH, et al. GDF15 Is an Inflammation-Induced Central Mediator of Tissue Tolerance. Cell. 2019 Aug 22;178(5):1231-1244.e1doi: 10.1016/j.cell.2019.07.033. Epub 2019 Aug 8. PMID: 31402172; PMCID: PMC6863354.

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GDF-15 Protein, Cynomolgus, Recombinant (His)

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