GDF-15 Protein, Rat, Recombinant (His)

Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15. GDF-15 Protein, Rat, Recombinant (His) is expressed in E. coli expression system with N-His tag. The predicted molecular weight is 13.68 kDa and the accession number is Q9Z0J6.

GDF-15 Protein, Rat, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 160,500
500 μg	約5 days	￥ 643,000
1 mg	約5 days	￥ 1,067,500

バルクのお問い合わせ

生物学的情報

Immobilized Rat GDF15, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Mouse GFRAL, His Tag with the EC50 of 0.95μg/ml determined by ELISA.

説明

Species	Rat
Expression Host	E. coli
Tag	N-His
Accession Number	Q9Z0J6
別名	GDF-15, PDF, PTGF-beta, MIC-1, MIC1, GDF15, PTGFBPTGF-beta, NAG-1, Placental TGF-β, PTGF-β, PLAB, PTGFBPTGF-β, Placental TGF-beta, PTGFB, RG-1
Construction	Ser189-Ala303
Protein Purity	> 95% as determined by Tris-Bis PAGE
分子量	13.68 kDa (predicted). The protein migrates to 15-17 kDa based on Tris-Bis PAGE result.

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM HAc (pH 2.9). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in 50mM HAc (pH 2.9). The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15.

参考文献

1. Baek SJ, Eling T. Growth differentiation factor 15 (GDF15): A survival protein with therapeutic potential in metabolic diseases. Pharmacol Ther. 2019 Jun;198:46-58. doi: 10.1016/j.pharmthera.2019.02.008. Epub 2019 Feb 18. PMID: 30790643; PMCID: PMC7196666.

Calculator

Reconstitution Calculator

Recombinant Proteins Dilute Calculator

Specific Activity Calculator

bottom

GDF-15 Protein, Rat, Recombinant (His)

参考文献

Calculator

技術サポート

Keywords