GM-CSFR alpha Protein, Human, Recombinant (hFc)

GM-CSFR alpha Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 61.2 kDa and the accession number is P15509-1.

GM-CSFR alpha Protein, Human, Recombinant (hFc)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 138,000
1 mg	約5 days	￥ 897,500

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説明	GM-CSFR alpha Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 61.2 kDa and the accession number is P15509-1.

Species	Human
Expression Host	HEK293 Cells
Tag	C-hFc
Accession Number	P15509-1
別名	CSF2RAX, CSF2R, CD116, CSF2RY, GM-CSF-R-α, GM-CSF-R-alpha, GM-CSF Receptor α Protein, colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage), CSF2RAY, GMCSFR, GMR, SMDP4, CSF2RX, CDw116, colony stimulating factor 2 receptor, α, low-affinity (granulocyte-macrophage)
Construction	The extracellular domain (Met 1-Gly 320) of Human GM-CSFRα (NP_006131.2) pro-protein was expressed with the C-terminal fused Fc region of Human IgG1.
Protein Purity	> 90 % as determined by SDS-PAGE
分子量	61.2 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	CD116/GM-CSFR has been preferentially associated with M4, M5 subtype of AML but is not specific. The cluster of differentiation (cluster of designation) (often abbreviated as CD) is a protocol used for the identification and investigation of cell surface molecules present on white blood cells initially but found in almost any kind of cell of the body, providing targets for immunophenotyping of cells. Physiologically, CD molecules can act in numerous ways, often acting as receptors or ligands (the molecule that activates a receptor) important to the cell. A signal cascade is usually initiated, altering the behavior of the cell (see cell signaling). Some CD proteins do not play a role in cell signaling, but have other functions, such as cell adhesion. CD116/GM-CSFR is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2, a cytokine which controls the production, differentiation, and function of granulocytes and macrophages. The encoded protein is a member of the cytokine family of receptors. CD116/GM-CSFR is found in the pseudoautosomal region (PAR) of the X and Y chromosomes.

参考文献

1. Sjöblom C,et al.(2002) Granulocyte-macrophage colony-stimulating factor (GM-CSF) acts independently of the beta common subunit of the GM-CSF receptor to prevent inner cell mass apoptosis in human embryos. Biol Reprod. 67(6): 1817-23. 2. Goldstein JI,et al.(2011) Defective leukocyte GM-CSF receptor (CD116) expression and function in inflammatory bowel disease. Gastroenterology. 141(1): 208-16. 3. Saulle E,et al.(2009) Colocalization of the VEGF-R2 and the common IL-3/GM-CSF receptor beta chain to lipid rafts leads to enhanced p38 activation. Br J Haematol. 145(3): 399-411.

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GM-CSFR alpha Protein, Human, Recombinant (hFc)

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