HDAC8 Protein, Human, Recombinant (GST)

HDAC8 Protein, Human, Recombinant (GST) is expressed in Baculovirus insect cells with GST tag. The predicted molecular weight is 68 kDa and the accession number is Q9BY41-1.

HDAC8 Protein, Human, Recombinant (GST)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 138,000

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生物学的情報	Testing in progress
説明	HDAC8 Protein, Human, Recombinant (GST) is expressed in Baculovirus insect cells with GST tag. The predicted molecular weight is 68 kDa and the accession number is Q9BY41-1.

Species	Human
Expression Host	Baculovirus Insect Cells
Tag	C-GST
Accession Number	Q9BY41-1
別名	histone deacetylase 8, CDA07, MRXS6, RPD3, CDLS5, WTS, HD8, HDACL1
Construction	The full length of Human HDAC8 isoform 1 (NP_060956.1) (Met 1-Val 377) was expressed, fused with the GST tag at the C-terminus.
Protein Purity	> 88 % as determined by SDS-PAGE
分子量	68 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 10% glycerol, pH 8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Histone deacetylase 8, also known as HDAC8 and HDACL1, is a nucleus and cytoplasm protein that belongs to the histone deacetylase family and HD type 1 subfamily. Histone deacetylases (HDACs) are a growing family of enzymes implicated in transcriptional regulation by affecting the acetylation state of core histones in the nucleus of cells. HDAC8 / HDACL1 is weakly expressed in most tissues. It is expressed at a higher level in the heart, brain, kidney, and pancreas and also in the liver, lung, placenta, prostate, and kidney. HDAC8 / HDACL1 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones ( H2A, H2B, H3, and H4 ). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression, and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC8 / HDACL1 may play a role in smooth muscle cell contractility. HDAC8 / HDACL1 may be a potential drug target for neuroblastoma differentiation therapy using selective inhibitors, avoiding unspecific side effects.

参考文献

1. Buggy JJ. et al.,2000, Biochem J. 350 (1): 199-205. 2. Krennhrubec K. et al., 2007, Bioorg Med Chem Lett. 17 (10): 2874-8. 3. Oehme I. et al., 2009, Expert Opin Investig Drugs.18 (11): 1605-17.

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HDAC8 Protein, Human, Recombinant (GST)

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