HtrA2/Omi Protein, Human, Recombinant (His)

カタログ番号 TMPY-02078

別名: PARK13, OMI, HtrA serine peptidase 2, PRSS25

Serine protease HTRA2, also known as high-temperature requirement protein A2, Omi stress-regulated endoprotease, Serine protease 25, Serine proteinase OMI and HTRA2, is a single-pass membrane protein that belongs to the peptidase S1B family. HTRA2 contains one PDZ (DHR) domain. HTRA2 is a serine protease that shows proteolytic activity against a non-specific substrate beta-casein. It promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity or by a BIRC inhibition-independent, caspase-independent, and serine protease activity-dependent mechanism. HTRA2 cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 of HTRA2 seems to be proteolytically inactive. Defects in HTRA2 are the cause of Parkinson disease type 13 (PARK13) which is a complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity, and postural instability, as well as by a clinically significant response to treatment with levodopa.

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HtrA2/Omi Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 138,000

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生物学的特性に関する説明

Technical Params

Product Properties

参考文献

生物学的情報

Protease activity demonstrated by HtrA2 cleavage of bovine β-casein. Incubation of β-casein at 0.2 mg/mL with Recombinant Human HTRA-2 at 0.02 mg/mL (ratio of 10:1) for 60 minutes at 45℃ in 50 mM Tris, pH 8.0, which results in >95% cleavage of β-casein, as revealed by SDS-PAGE.

説明

Species	Human
Expression Host	E. coli
Tag	His
Accession Number	O43464-1
別名	PARK13, OMI, HtrA serine peptidase 2, PRSS25
Construction	A DNA sequence encoding the mature form of human HTRA2 (O43464-1) (Ala 134-Glu 458) was expressed, with a polyhistide tag at the C-terminus.
Protein Purity	> 87 % as determined by SDS-PAGE
分子量	36.5 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Supplied as sterile 50mM Tris, 0. 3M NaCl, 1mM DTT, 20% Glycerol, pH 7.8Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	Solution. It is shipped out with blue ice.
Research Background	Serine protease HTRA2, also known as high-temperature requirement protein A2, Omi stress-regulated endoprotease, Serine protease 25, Serine proteinase OMI and HTRA2, is a single-pass membrane protein that belongs to the peptidase S1B family. HTRA2 contains one PDZ (DHR) domain. HTRA2 is a serine protease that shows proteolytic activity against a non-specific substrate beta-casein. It promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity or by a BIRC inhibition-independent, caspase-independent, and serine protease activity-dependent mechanism. HTRA2 cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 of HTRA2 seems to be proteolytically inactive. Defects in HTRA2 are the cause of Parkinson disease type 13 (PARK13) which is a complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity, and postural instability, as well as by a clinically significant response to treatment with levodopa.

参考文献

1. Faccio L., et al., 2000, J. Biol. Chem. 275:2581-2588. 2. Gray C.W., et al., 2000, Eur. J. Biochem. 267:5699-5710. 3. Suzuki Y., et al., 2001, Mol. Cell 8:613-621. 4. Strauss K.M., et al., 2005, Hum. Mol. Genet. 14:2099-2111.

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Keywords

HtrA2/Omi Protein, Human, Recombinant (His) PARK-13 PARK13 OMI PRSS-25 HtrA serine peptidase 2 PARK 13 PRSS25 PRSS 25 recombinant recombinant-proteins proteins protein