IL-12 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 57.2 kDa and the accession number is P29459-1&NP_002178.2.
| 生物学的情報 |
Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.2-2 ng/mL.
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| 説明 |
IL-12 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 57.2 kDa and the accession number is P29459-1&NP_002178.2. |
| Species |
Human
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| Expression Host |
HEK293 Cells
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| Tag |
Tag Free
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| Accession Number |
P29459-1&NP_002178.2
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| 別名 |
IMD29, CLMF, IL12, NFSK, IL-12A, interleukin 12A, NKSF1, IL12 p70, IL-12B, P35, NKSF2, CLMF2, NKSF, IMD28
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| Construction |
The Human IL12A (P29459-1)(Met1-Ser219) was expressed, constructed the plasmid 1; The Human IL12B (NP_002178.2)(Met1-Ser328) was expressed, constructed the plasmid 2. The two plasmids were co-expressed and the Human IL12A&IL12B heterodimer was purified.
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| Protein Purity |
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
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| 分子量 |
57.2 kDa (predicted)
|
| Endotoxin |
< 5 EU/mg of the protein.
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| Formulation |
Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
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| Reconstitution |
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
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| Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
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| Shipping |
In general, Lyophilized powders are shipping with blue ice.
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