IL-32 Protein, Human, Recombinant (isoform alpha, His)

カタログ番号 TMPY-01033

別名: IL-32δ, IL-32beta, IL32, TAIFc, IL-32γ, IL-32 Protein(isoform α), IL-32alpha, IL-32delta, TAIFb, TAIF, NK4, IL-32gamma, interleukin 32, TAIFa, IL-32β, TAIFd, IL-32α

IL-32 is a recently discovered cytokine that induces various proinflammatory cytokines (TNF-alpha, IL-1beta, IL-6) and chemokines in both human and mouse cells through the NF-kappaB and p38 MAPK inflammatory signal pathways. It is regulated robustly by other major proinflammatory cytokines and is crucial to inflammation and immune responses. Four of the IL-32 isoforms (alpha, beta, gamma, and delta) are the most representative IL-32 transcripts, and the gamma isoform of IL-32 is the most active, although all isoforms are biologically active. IL-32, a cytokine produced mainly by T, natural killer, and epithelial cells induces significant amounts of TNFalpha and MIP-2 and increases the production of both cytokines in a dose-dependent manner. IL-32 has been implicated in inflammatory disorders, Mycobacterium tuberculosis infections, inflammatory bowel disease, and influenza A virus infection, as well as in some autoimmune diseases, such as rheumatoid arthritis, ulcerative colitis, and in the human stomach cancer, human lung cancer, and breast cancer tissues. Thus, IL-32 expression might be valuable as a biomarker for cancer.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

IL-32 Protein, Human, Recombinant (isoform alpha, His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 106,000
1 mg	約5 days	￥ 688,500

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Product Properties

参考文献

説明

Species	Human
Expression Host	HEK293 Cells
Tag	C-His
Accession Number	P24001-4
別名	IL-32δ, IL-32beta, IL32, TAIFc, IL-32γ, IL-32 Protein(isoform α), IL-32alpha, IL-32delta, TAIFb, TAIF, NK4, IL-32gamma, interleukin 32, TAIFa, IL-32β, TAIFd, IL-32α
Construction	The Human IL32 isoform alpha (IL32A) (NP_001012651.1) precursor (Met 1-Lys 131) was expressed with a polyhistidine tag at the C-terminus.
Protein Purity	> 95 % as determined by SDS-PAGE
分子量	15.7 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	IL-32 is a recently discovered cytokine that induces various proinflammatory cytokines (TNF-alpha, IL-1beta, IL-6) and chemokines in both human and mouse cells through the NF-kappaB and p38 MAPK inflammatory signal pathways. It is regulated robustly by other major proinflammatory cytokines and is crucial to inflammation and immune responses. Four of the IL-32 isoforms (alpha, beta, gamma, and delta) are the most representative IL-32 transcripts, and the gamma isoform of IL-32 is the most active, although all isoforms are biologically active. IL-32, a cytokine produced mainly by T, natural killer, and epithelial cells induces significant amounts of TNFalpha and MIP-2 and increases the production of both cytokines in a dose-dependent manner. IL-32 has been implicated in inflammatory disorders, Mycobacterium tuberculosis infections, inflammatory bowel disease, and influenza A virus infection, as well as in some autoimmune diseases, such as rheumatoid arthritis, ulcerative colitis, and in the human stomach cancer, human lung cancer, and breast cancer tissues. Thus, IL-32 expression might be valuable as a biomarker for cancer.

参考文献

1. Felaco P, et al. (2009) IL-32: a newly-discovered proinflammatory cytokine. J Biol Regul Homeost Agents. 23(3): 141-7. 2. Kobayashi H, et al. (2009) Molecular characterization of IL-32 in human endothelial cells. Cytokine. 46(3): 351-8. 3. Meyer N, et al. (2010) IL-32 is expressed by human primary keratinocytes and modulates keratinocyte apoptosis in atopic dermatitis. J Allergy Clin Immunol. 125(4): 858-865.

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Keywords

IL-32 Protein, Human, Recombinant (isoform alpha, His) IL-32 IL-32δ IL 32 IL-32beta IL32 TAIFc IL-32γ IL-32 Protein(isoform α) NK 4 IL-32alpha IL-32delta TAIFb TAIF NK4 IL-32gamma interleukin 32 NK-4 TAIFa IL-32β TAIFd IL-32α recombinant recombinant-proteins proteins protein