IL-4 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 18 KDa and the accession number is P05112.
| 生物学的情報 |
1. Loaded Cynomolgus IL-4RA-Fc on Protein A Biosensor, can bind Human IL-4-His with an affinity constant of 0.33 nM as determined in BLI assay. (Regularly tested).
2. Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.05-0.5 ng/ml. (QC verified);
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| 説明 |
IL-4 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 18 KDa and the accession number is P05112. |
| Species |
Human
|
| Expression Host |
HEK293 Cells
|
| Tag |
C-6xHis
|
| Accession Number |
P05112
|
| 別名 |
Pitrakinra, Binetrakin, B-Cell Stimulatory Factor 1, IL4, Interleukin-4, IL-4, BSF-1, Lymphocyte Stimulatory Factor 1
|
| Amino Acid |
His25-Ser153
|
| Construction |
His25-Ser153
|
| Protein Purity |
Greater than 95% as determined by reducing SDS-PAGE. Greater than 95% as determined by SEC-HPLC.
|
| 分子量 |
18 KDa, reducing conditions
|
| Endotoxin |
< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
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| Formulation |
Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4.
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| Reconstitution |
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
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| Stability & Storage |
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
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| Shipping |
In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
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| Research Background |
Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. IL-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergic response.
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