IL-5 Protein, Human, Recombinant

IL-5 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 13.1 kDa and the accession number is P05113.

IL-5 Protein, Human, Recombinant

パッケージサイズ	在庫状況	単価(税別)
5 μg	在庫あり	￥ 13,000
10 μg	約5 days	￥ 21,500
20 μg	約5 days	￥ 34,000
50 μg	約5 days	￥ 64,000
100 μg	約5 days	￥ 102,500

バルクのお問い合わせ

生物学的情報	Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-0.5 ng/mL.
説明	IL-5 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 13.1 kDa and the accession number is P05113.

Species	Human
Expression Host	E. coli
Tag	Tag Free
Accession Number	P05113
別名	TRF, EDF, interleukin 5, IL-5
Construction	A DNA sequence encoding the human IL5 (P05113) (Ile20-Ser134) was expressed.
Protein Purity	> 95 % as determined by SDS-PAGE
分子量	13.1 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Interleukin 5 (IL-5) is a member of the interleukin family with a length of 115 amino acids. Interleukins are a group of cytokines (secreted proteins/signaling molecules) that were first seen to be expressed by white blood cells (leukocytes) and has been found in a wide variety of body cells. Interleukin 5 or IL-5 is produced by T helper-2 cells and mast cells. It helps to stimulate B cell growth and increase immunoglobulin secretion and is considered a key mediator in eosinophil activation. Interleukin 5 (IL-5) has long been associated with several allergic diseases, including allergic rhinitis and asthma. Growth in the number of circulating, airway tissue, and induced sputum eosinophils have been observed in patients with these diseases. IL-5 also had something with the terminally differentiated granulocyte eosinophils. IL-5 was originally found as an eosinophil colony-stimulating factor. It has been proved to be a major regulator of eosinophil accumulation in tissues and can modulate eosinophil behavior at every stage from maturation to survival.

参考文献

1. Milburn MV,et al.(1993) A novel dimer configuration revealed by the crystal structure at 2.4 A resolution of human interleukin-5. Nature. 363(6425): 172-176. 2. Lee JS,et al.(1989) The IL-4 and IL-5 genes are closely linked and are part of a cytokine gene cluster on mouse chromosome 11. Somat Cell Mol Genet. 15(2): 143-152. 3. Woodcock JM,et al.(1994) Three residues in the common beta chain of the human GM-CSF, IL-3 and IL-5 receptors are essential for GM-CSF and IL-5 but not IL-3 high affinity binding and interact with Glu21 of GM-CSF. EMBO J. 13 (21): 5176-85.

Calculator

Reconstitution Calculator

Recombinant Proteins Dilute Calculator

Specific Activity Calculator

bottom

IL-5 Protein, Human, Recombinant

参考文献

Calculator

技術サポート

Keywords