Integrin alpha V beta 6 Protein, Human, Recombinant (Flag & His)

Integrin alpha V beta 6 Protein, Human, Recombinant (Flag & His) is expressed in HEK293 mammalian cells with Flag and His tag. The predicted molecular weight is 190.4 kDa and the accession number is P06756-1&P18564-1.

Integrin alpha V beta 6 Protein, Human, Recombinant (Flag & His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 119,000
200 μg	約5 days	￥ 209,500
500 μg	約5 days	￥ 443,000

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生物学的情報	Testing in progress
説明	Integrin alpha V beta 6 Protein, Human, Recombinant (Flag & His) is expressed in HEK293 mammalian cells with Flag and His tag. The predicted molecular weight is 190.4 kDa and the accession number is P06756-1&P18564-1.

Species	Human
Expression Host	HEK293 Cells
Tag	Flag, His
Accession Number	P06756-1&P18564-1
別名	Integrin alpha V β6, ITGAV & ITGB6, integrin, alpha 5 (fibronectin receptor, alpha polypeptide), Integrin α V β 6, integrin, α5 (fibronectin receptor, α polypeptide)
Construction	The extracellular domain (Met 1-Val992) of Human ITGAV (P06756-1) was fused with a flag tag at the C-terminus, constructed the plasmid 1; The extracellular domain (Met1-Asn707) of Human ITGB6 (P18564-1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the Human ITGAV&ITGB6 heterodimer was purified.
Protein Purity	> 95 % as determined by SDS-PAGE
分子量	190.4 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Integrin alpha-5, also known as ITGA5, is a single-pass type I membrane protein which belongs to the integrin alpha chain family. ITGA5 contains 7 FG-GAP repeats. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. ITGAV&ITGB6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of ITGAV&ITGB6 via clathrin-mediated endocytosis promotes carcinoma cell invasion.

参考文献

1. Suzuki S.,et al.,(1987), Amino acid sequence of the vitronectin receptor alpha subunit and comparative expression of adhesion receptor mRNAs. J. Biol. Chem. 262:14080-14085. 2. Sims M.A.,et al., (2000), Cloning and characterisation of ITGAV, the genomic sequence for human cell adhesion protein (vitronectin) receptor alpha subunit, CD51.Cytogenet. Cell Genet. 89:268-271. 3. Ota T.,et al.,(2004), Complete sequencing and characterization of 21,243 full-length human cDNAs.Nat. Genet. 36:40-45.

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Integrin alpha V beta 6 Protein, Human, Recombinant (Flag & His)

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