IRE1 Protein, Human, Recombinant (aa 465-977, His & GST)

IRE1 Protein, Human, Recombinant (aa 465-977, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 86 kDa and the accession number is O75460-1.

IRE1 Protein, Human, Recombinant (aa 465-977, His & GST)

パッケージサイズ	在庫状況	単価(税別)
50 μg	在庫あり	￥ 73,500
100 μg	約5 days	￥ 125,500
200 μg	約5 days	￥ 214,500
500 μg	約5 days	￥ 434,000

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生物学的情報	The specific activity was determined to be 5 nmol/min/mg using MBP as substrate
説明	IRE1 Protein, Human, Recombinant (aa 465-977, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 86 kDa and the accession number is O75460-1.

Species	Human
Expression Host	Baculovirus-Insect Cells
Tag	His,GST
Accession Number	O75460-1
別名	hIRE1p, endoplasmic reticulum to nucleus signaling 1, IRE1P, IRE1a, IRE1
Construction	A DNA sequence encoding the human ERN1 isoform 1 (O75460-1) cytoplasmic domain (Pro 465-Leu 977) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Protein Purity	> 80 % as determined by SDS-PAGE
分子量	86 kDa (predicted)

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Supplied as sterile 20mM Tris, 500M NaCl, 25% glycerol, 0. 1mM EDTA, 0. 5mM TCEP, 2mM GSH, pH 7.5. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Research Background	Endoplasmic reticulum stress and hypoxia are necessary components of malignant tumors growth and suppression of ERN1 (from endoplasmic reticulum to nuclei-1) signalling pathway, which is linked to the apoptosis and cell death processes, significantly decreases proliferative processes. An enhanced expression of TP53 gene in ERN1 knockdown glioma cells correlates with the decreased level of ubiquitin ligase MDM2 and increased expression level of USP7 which deubiquitinates TP53 and MDM2 and induces TP53-dependent cell growth repression and apoptosis. Thus, the expression of genes encoding TP53 and related to TP53 factors depends upon the endoplasmic reticulum stress signaling as well as on hypoxia, and correlates with suppression of glioma growth under ERN1 knockdown. The dependence of insulin-like growth binding proteins as well as IGF2BP3 and HTRA1 gene expressions in U87 glioma cells on ERN1 signaling enzyme function and hypoxia, indicating its participation in the regulation of metabolic and proliferative processes via IGF/INS receptors, because endoplasmic reticulum stress is an important component of tumor growth and metabolic diseases.

参考文献

1. Trentmann,S.M. et al., 2000, Plant Mol Biol. 44 (1):11-25. 2. Iwawaki T., et al., 2001, Nat. Cell Biol. 3:158-64. 3. Liu C.Y., et al., 2003, J. Biol. Chem. 278:17680-7. 4. Oppermann F.S., et al., 2009, Mol. Cell. Proteomics 8:1751-64.

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IRE1 Protein, Human, Recombinant (aa 465-977, His & GST)

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