ITK Protein, Human, Recombinant (Actived By LCK, GST)

ITK Protein, Human, Recombinant (Actived By LCK, GST) is expressed in Baculovirus insect cells with GST tag. The predicted molecular weight is 57.7 kDa and the accession number is Q08881.

ITK Protein, Human, Recombinant (Actived By LCK, GST)

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 114,500

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説明	ITK Protein, Human, Recombinant (Actived By LCK, GST) is expressed in Baculovirus insect cells with GST tag. The predicted molecular weight is 57.7 kDa and the accession number is Q08881.

Species	Human
Expression Host	Baculovirus Insect Cells
Tag	N-GST
Accession Number	Q08881
別名	LYK, PSCTK2, LPFS1, IL2-inducible T-cell kinase, EMT
Construction	The Human ITK (NP_005537.3) (Arg352-Leu620) was expressed with a GST tag at the N-terminus. The purified ITK was actived by LCK.
Protein Purity	> 75 % as determined by SDS-PAGE.
分子量	57.7 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Supplied as sterile 50 mM Tris, 150 mM NaCl, pH 7.5, 25% glycerol, 0.5 mM PMSF, 0.5 mM TCEP, 0.1 mM EDTA, 0.5 mM GSH.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background	IL-2-inducible T cell kinase is a member of the protein kinase superfamily, Tyr protein kinase family, and TEC subfamily. It contains 1 Btk-type zinc finger, 1 PH domain, 1 protein kinase domain, 1 SH2 domain, and 1 SH3 domain. As an intracellular kinase expressed in T-cells, IL-2-inducible T cell kinase contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. It regulates the development, function, and differentiation of conventional T-cells and nonconventional NKT-cells. IL-2-inducible T cell kinase also plays an essential role in the regulation of the adaptive immune response. Effects in IL-2-inducible T cell kinase are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1). LPSA1 is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Inadequate immune response to EBV can have a fatal outcome. Clinical features include splenomegaly, lymphadenopathy, anemia, thrombocytopenia, pancytopenia, recurrent infections. There is an increased risk of lymphoma.

参考文献

1. Lee SH,et al. (2011) The association of a single-nucleotide polymorphism of the IL-2 inducible T-cell Kinase gene with asthma. Ann Hum Genet. 75(3):359-69. 2. Yao HL,et al. (2010) Effect of Itk down regulation on cytokines production in Jurkat cell. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 24(5):358-61. 3. Pechloff K,et al. (2010) The fusion kinase ITK-SYK mimics a T cell receptor signal and drives oncogenesis in conditional mouse models of peripheral T cell lymphoma. J Exp Med. 207(5):1031-44.

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ITK Protein, Human, Recombinant (Actived By LCK, GST)

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