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MSH2 Protein, Human, Recombinant (His & GST)

カタログ番号 TMPY-04267
別名: FCC1, COCA1, HNPCC1, HNPCC, LCFS2, mutS homolog 2

MSH2 is a key DNA mismatch repair protein, which plays an important role in genomic stability. In addition to its DNA repair function, MSH2 serves as a sensor for DNA base analogs-provoked DNA replication errors and binds to various DNA damage-induced adducts to trigger cell cycle arrest or apoptosis. Loss or depletion of MSH2 from cells renders resistance to certain DNA-damaging agents. Therefore, the level of MSH2 determines the DNA damage response.MSH2 is a central component of the mismatch repair pathway that targets mismatches arising during DNA replication, homologous recombination (HR), and in response to genotoxic stresses.MSH2 rearrangements are involved in approximately 10% of hereditary non-polyposis colorectal cancer (HNPCC) families, and in most of the rearrangements, exon 1 is deleted. Loss of human MSH2 (hMSH2) protein might be involved in the multistep pathogenesis of hematological malignancies associated with genetic instability.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
MSH2 Protein, Human, Recombinant (His & GST)
パッケージサイズ 在庫状況 単価(税別)
100 μg 約5 days ¥ 161,000
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生物学的特性に関する説明
Technical Params
Product Properties
説明 MSH2 is a key DNA mismatch repair protein, which plays an important role in genomic stability. In addition to its DNA repair function, MSH2 serves as a sensor for DNA base analogs-provoked DNA replication errors and binds to various DNA damage-induced adducts to trigger cell cycle arrest or apoptosis. Loss or depletion of MSH2 from cells renders resistance to certain DNA-damaging agents. Therefore, the level of MSH2 determines the DNA damage response.MSH2 is a central component of the mismatch repair pathway that targets mismatches arising during DNA replication, homologous recombination (HR), and in response to genotoxic stresses.MSH2 rearrangements are involved in approximately 10% of hereditary non-polyposis colorectal cancer (HNPCC) families, and in most of the rearrangements, exon 1 is deleted. Loss of human MSH2 (hMSH2) protein might be involved in the multistep pathogenesis of hematological malignancies associated with genetic instability.
Species Human
Expression Host Baculovirus-Insect Cells
Tag His,GST
Accession Number P43246-1
別名 FCC1, COCA1, HNPCC1, HNPCC, LCFS2, mutS homolog 2
Construction A DNA sequence encoding the human MSH2 (NP_000242.1) (Met1-Thr934) was expressed with a C-terminal polyhistidine-tagged GST tag at the N-terminus (his-GST).
Protein Purity > 90 % as determined by SDS-PAGE.
分子量 132.6 kDa (predicted)
Endotoxin < 1.0 EU per μg protein as determined by the LAL method.
Formulation Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol, 20 mM GSH. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Research Background MSH2 is a key DNA mismatch repair protein, which plays an important role in genomic stability. In addition to its DNA repair function, MSH2 serves as a sensor for DNA base analogs-provoked DNA replication errors and binds to various DNA damage-induced adducts to trigger cell cycle arrest or apoptosis. Loss or depletion of MSH2 from cells renders resistance to certain DNA-damaging agents. Therefore, the level of MSH2 determines the DNA damage response.MSH2 is a central component of the mismatch repair pathway that targets mismatches arising during DNA replication, homologous recombination (HR), and in response to genotoxic stresses.MSH2 rearrangements are involved in approximately 10% of hereditary non-polyposis colorectal cancer (HNPCC) families, and in most of the rearrangements, exon 1 is deleted. Loss of human MSH2 (hMSH2) protein might be involved in the multistep pathogenesis of hematological malignancies associated with genetic instability.

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Please read the User Guide of Recombinant Proteins for more specific information.

Keywords

MSH2 Protein, Human, Recombinant (His & GST) FCC 1 FCC1 COCA1 HNPCC-1 HNPCC1 COCA 1 FCC-1 HNPCC 1 HNPCC LCFS2 LCFS-2 mutS homolog 2 LCFS 2 COCA-1 recombinant recombinant-proteins proteins protein