Nectin-4 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 35.6 kDa and the accession number is K4PZ75.
| 生物学的情報 |
Measured by its binding ability in a functional ELISA. Immobilized Human NECTIN4 His at 2 μg/ml (100 μl/well) can bind Human PVRL1 His , biotinylated, the EC50 of Human PVRL1 His, biotinylated is 200-800 ng/mL.
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| 説明 |
Nectin-4 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 35.6 kDa and the accession number is K4PZ75. |
| Species |
Human
|
| Expression Host |
HEK293 Cells
|
| Tag |
C-His
|
| Accession Number |
K4PZ75
|
| 別名 |
EDSS1, LNIR, nectin-4, PVRL4, PRR4
|
| Construction |
The Human NECTIN4 (NP_112178.2) (Met1-Ser349) was expressed with a polyhistidine tag at the C-terminus.
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| Protein Purity |
>95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
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| 分子量 |
35.6 kDa (predicted)
|
| Endotoxin |
< 1.0 EU/μg of the protein as determined by the LAL method.
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| Formulation |
Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
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| Reconstitution |
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
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| Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
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| Shipping |
In general, Lyophilized powders are shipping with blue ice.
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