NELL2 Protein, Human, Recombinant (His)

A brain-enriched secreting signal peptide, NELL2, has been suggested to play multiple roles in the development, survival, and activity of neurons in the mammal. NELL2 is an abundant glycoprotein containing an EGF-like domain in the neural tissues where it has multiple physiological functions by interacting with protein kinase C (PKC). There are two different splicing variant forms of NELL2 identified so far. One is secreted NELL2 (sNELL2) which is a neuron-specific variant and the other is cytosolic NELL2 (cNELL2) which is a non-secreted splicing variant of NELL2. NELL2 is strongly expressed in the brain of adults and fetuses but only weakly in the fetal kidney.

NELL2 Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 138,000

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説明

Species	Human
Expression Host	Baculovirus Insect Cells
Tag	His
Accession Number	Q99435
別名	NEL-like 2 (chicken), NRP2
Construction	The Human NELL2 (Q99435) (Met 1-Leu 816) was fused with a polyhistidine tag at the C-terminus.
Protein Purity	> 87 % as determined by SDS-PAGE
分子量	90.4 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, pH 7.4, 10% gly. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	A brain-enriched secreting signal peptide, NELL2, has been suggested to play multiple roles in the development, survival, and activity of neurons in the mammal. NELL2 is an abundant glycoprotein containing an EGF-like domain in the neural tissues where it has multiple physiological functions by interacting with protein kinase C (PKC). There are two different splicing variant forms of NELL2 identified so far. One is secreted NELL2 (sNELL2) which is a neuron-specific variant and the other is cytosolic NELL2 (cNELL2) which is a non-secreted splicing variant of NELL2. NELL2 is strongly expressed in the brain of adults and fetuses but only weakly in the fetal kidney.

参考文献

1. Kuroda S,et al.(1999) Biochemical characterization and expression analysis of neural thrombospondin-1-like proteins NELL1 and NELL2. Biochem Biophys Res Commun. 265(1): 79-86. 2. Oyasu M,et al.(2000) Immunocytochemical localization of a neuron-specific thrombospondin-1-like protein, NELL2: light and electron microscopic studies in the rat brain. Brain Res Mol Brain Res. 76(1): 151-60. 3. Nelson BR,et al.(2002) Restricted neural epidermal growth factor-like like 2 (NELL2) expression during muscle and neuronal differentiation. Mech Dev. 119 Suppl 1: 11-9.

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NELL2 Protein, Human, Recombinant (His)

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