p38 Protein, Human, Recombinant (His)

カタログ番号 TMPY-04260

別名: mitogen-activated protein kinase 14, CSPB1, PRKM14, RK, p38α, CSBP1, SAPK2A, CSBP2, p38, EXIP, Mxi2, PRKM15, CSBP, p38ALPHA

MAPK14 contains 1 protein kinase domain and belongs to the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. MAPK14 can be detected in the brain, heart, placenta, pancreas, and skeletal muscle and it is expressed to a lesser extent in the lung, liver, and kidney. MAPK14 is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with MAPK14. The substrates of p38 alpha include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of p38 alpha in stress-related transcription and cell cycle regulation, as well as in genotoxic stress response. In response to activation by environmental stress, pro-inflammatory cytokines, and lipopolysaccharide, MAPK14 phosphorylates some transcription factors, such as ELK1 and ATF2, and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. MAPK14 plays a critical role in the production of some cytokines, for example, IL-6. It may play a role in the stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2.

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p38 Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
20 μg	約5 days	￥ 138,000

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生物学的特性に関する説明

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Product Properties

参考文献

説明

Species	Human
Expression Host	Baculovirus Insect Cells
Tag	His
Accession Number	L7RSM2
別名	mitogen-activated protein kinase 14, CSPB1, PRKM14, RK, p38α, CSBP1, SAPK2A, CSBP2, p38, EXIP, Mxi2, PRKM15, CSBP, p38ALPHA
Construction	The amino acids cprresponding to the full length of Human MAPK14 isoform 1 (NP_001306.1) (Met1-Ser360) was fused with a polyhistidine tag at the N-terminus. Activated in vitro by MAP2K6 (10422-H20B1).
Protein Purity	≥ 85 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
分子量	43.7 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Supplied as sterile 50 mM Tris-HCl, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol, pH 7.5.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background	MAPK14 contains 1 protein kinase domain and belongs to the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. MAPK14 can be detected in the brain, heart, placenta, pancreas, and skeletal muscle and it is expressed to a lesser extent in the lung, liver, and kidney. MAPK14 is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with MAPK14. The substrates of p38 alpha include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of p38 alpha in stress-related transcription and cell cycle regulation, as well as in genotoxic stress response. In response to activation by environmental stress, pro-inflammatory cytokines, and lipopolysaccharide, MAPK14 phosphorylates some transcription factors, such as ELK1 and ATF2, and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. MAPK14 plays a critical role in the production of some cytokines, for example, IL-6. It may play a role in the stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2.

参考文献

1. Luo X,et al.(2011) Study on p38 mitogen activated protein kinase in vascular endothelial cells dysfunction in preeclampsia. Zhonghua Fu Chan Ke Za Zhi. 46(1):36-40. 2. Park CH,et al.(2011) Epidermal growth factor-induced matrix metalloproteinase-1 expression is negatively regulated by p38 MAPK in human skin fibroblasts. J Dermatol Sci. 64(2):134-41. 3. Lee JY,et al.(2011) Curcumin induces EGFR degradation in lung adenocarcinoma and modulates p38 activation in intestine: the versatile adjuvant for gefitinib therapy. PLoS One. 6(8):e23756. 4. Riis JL,et al.(2011) CCL27 expression is regulated by both p38 MAPK and IKKβ signalling pathways. Cytokine. 56(3):699-707.

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Keywords

p38 Protein, Human, Recombinant (His) mitogen-activated protein kinase 14 CSPB1 PRKM14 CSBP 1 CSBP 2 RK CSPB 1 p38α CSBP1 SAPK2A CSBP2 p38 EXIP CSBP-1 Mxi2 PRKM 14 PRKM 15 CSBP-2 PRKM15 PRKM-14 CSPB-1 CSBP PRKM-15 p38ALPHA recombinant recombinant-proteins proteins protein