PHGDH Protein, Human, Recombinant (His)

カタログ番号 TMPY-02801

別名: phosphoglycerate dehydrogenase, HEL-S-113, 3PGDH, NLS1, PHGDHD, PGDH, NLS, SERA, PGAD, 3-PGDH, PGD, PDG

PHGDH is a member of the D-isomer specific 2-hydroxyacid dehydrogenase family. This new family consists of D-isomer-stereospecific enzymes. The conserved residues in this family appear to be the residues involved in the substrate binding and the catalytic reaction, and thus to be targets for site-directed mutagenesis. A number of NAD-dependent 2-hydroxyacid dehydrogenases which seem to be specific for the D-isomer of their substrate have been shown to be functionally and structurally related. PHGDH catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor. Overexpression of PHGDH may cause certain breast cancers. Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency which is characterized by congenital microcephaly, psychomotor retardation, and seizures.

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PHGDH Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 98,500
200 μg	約5 days	￥ 168,000
500 μg	約5 days	￥ 340,000

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COA DATASHEET SDS

生物学的特性に関する説明

Technical Params

Product Properties

参考文献

生物学的情報

Testing in progress

説明

Species	Human
Expression Host	E. coli
Tag	His
Accession Number	O43175
別名	phosphoglycerate dehydrogenase, HEL-S-113, 3PGDH, NLS1, PHGDHD, PGDH, NLS, SERA, PGAD, 3-PGDH, PGD, PDG
Construction	A DNA sequence encoding the mature form of human PHGDH (O43175) (Met 1-Phe 533) was fused with a polyhistidine tag at the C-terminus and an initial Met at the N-terminus.
Protein Purity	> 90 % as determined by SDS-PAGE
分子量	58kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Supplied as sterile PBS, 100mM Arg, 0. 1% Tween20, 20% glycerol, pH 8.0. Pleasecon tact usfor any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	Solution. It is shipped out with blue ice.
Research Background	PHGDH is a member of the D-isomer specific 2-hydroxyacid dehydrogenase family. This new family consists of D-isomer-stereospecific enzymes. The conserved residues in this family appear to be the residues involved in the substrate binding and the catalytic reaction, and thus to be targets for site-directed mutagenesis. A number of NAD-dependent 2-hydroxyacid dehydrogenases which seem to be specific for the D-isomer of their substrate have been shown to be functionally and structurally related. PHGDH catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor. Overexpression of PHGDH may cause certain breast cancers. Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency which is characterized by congenital microcephaly, psychomotor retardation, and seizures.

参考文献

1. Pind S,et al. (2002) V490M, a common mutation in 3-phosphoglycerate dehydrogenase deficiency, causes enzyme deficiency by decreasing the yield of mature enzyme. J Biol Chem. 277 (9): 7136-43. 2. Du H,et al. (2010) 3-Phosphoglycerate dehydrogenase expression is regulated by HOXA10 in murine endometrium and human endometrial cells. Reproduction. 139 (1): 237-45. 3. Possemato R,et al. (2011) Functional genomics reveal that the serine synthesis pathway is essential in breast cancer. Nature. 476 (7360): 346-50.

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Keywords

PHGDH Protein, Human, Recombinant (His) NLS-1 phosphoglycerate dehydrogenase HEL-S-113 3PGDH NLS1 PHGDHD HEL-S 113 HEL-S113 PGDH NLS SERA PGAD NLS 1 3-PGDH PGD PDG recombinant recombinant-proteins proteins protein