PKM2 Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 59 kDa and the accession number is P14618-1.
説明 | PKM2 Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 59 kDa and the accession number is P14618-1. |
Species | Human |
Expression Host | E. coli |
Tag | His |
Accession Number | P14618-1 |
別名 | pyruvate kinase, muscle, OIP3, CTHBP, PK3, THBP1, HEL-S-30, TCB, PKM2 |
Construction | The Human PKM2 isoform M2 (P14618-1) (Ser 2-Pro 531) was expressed, with a polyhistide tag at the N-terminus. |
Protein Purity | > 90 % as determined by SDS-PAGE |
分子量 | 59 kDa (predicted) |
Endotoxin | Please contact us for more information. |
Formulation | Supplied as sterile PBS, pH 7.0, 10% glycerol. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. |
Research Background | Pyruvate kinase isozymes M2 also known as pyruvate kinase muscle isozyme 2 (PKM2), pyruvate kinase type K, cytosolic thyroid hormone-binding protein (CTHBP), thyroid hormone-binding protein 1 (THBP1), or opa-interacting protein 3 (OIP3), is an isoenzyme of the glycolytic enzyme pyruvate kinase. Pyruvate kinase isozymes M2 / PKM2 is a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. PKM2 has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. PKM2 has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. PKM2 functions as a glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. PKM2 may stimulate POU5F1-mediated transcriptional activation. This protein plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms of PKM2 contributes to the control of glycolysis and is important for tumor cell proliferation and survival. |
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Please read the User Guide of Recombinant Proteins for more specific information.
PKM2 Protein, Human, Recombinant (His) pyruvate kinase, muscle THBP-1 PK-3 PK 3 OIP3 OIP 3 CTHBP PK3 PKM-2 THBP1 HEL-S-30 THBP 1 OIP-3 TCB PKM2 PKM 2 recombinant recombinant-proteins proteins protein