Renin Protein, Human, Recombinant (R66K, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 43.74 kDa and the accession number is P00797-1.
説明 | Renin Protein, Human, Recombinant (R66K, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 43.74 kDa and the accession number is P00797-1. |
Species | Human |
Expression Host | HEK293 Cells |
Tag | C-His |
Accession Number | P00797-1 |
別名 | REN, FLJ10761, renin, HNFJ2 |
Construction | The Human REN (NP_000528.1, with mutation Arg 66 Lys) (Met1-Arg406) |
Protein Purity | > 95 % as determined by SDS-PAGE. |
分子量 | 43.74 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM Tris, 150 mM NaCl, pH 8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | Renin-1, also known as Ren-1, Angiotensinogenase and Kidney renin, is a member of the peptidase A1 family. Renin-1 is synthesized by the juxtaglomerular cells of the kidney in response to decreased blood pressure and sodium concentration. androgen and thyroid hormones influence levels of Renin-1 in mouse submandibular gland (SMG) primarily by regulating the amount of Renin-1 mRNA available for translation. Renin-1 is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. It is expressed at relatively low levels in mouse SMG and kidney. Ren-2 is expressed at high levels in the mouse SMG and at very low levels, if at all, in the kidney. Ren-1 and Ren-2 are closely linked on mouse chromosome 1, show extensive homology in coding and noncoding regions and provide a model for studying the regulation of gene expression. |
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Renin Protein, Human, Recombinant (R66K, His) REN FLJ10761 renin HNFJ2 HNFJ 2 HNFJ-2 FLJ 10761 FLJ-10761 recombinant recombinant-proteins proteins protein