Src Protein, Human, Recombinant (His & GST)

Src Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 87.7 kDa and the accession number is P12931-1.

Src Protein, Human, Recombinant (His & GST)

パッケージサイズ	在庫状況	単価(税別)
50 μg	在庫あり	￥ 73,500
100 μg	約5 days	￥ 125,500
200 μg	約5 days	￥ 214,500
500 μg	約5 days	￥ 434,000

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生物学的情報	The specific activity was determined to be ＞80 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.
説明	Src Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 87.7 kDa and the accession number is P12931-1.

Species	Human
Expression Host	Baculovirus Insect Cells
Tag	His, GST
Accession Number	P12931-1
別名	c-SRC, p60-Src, ASV, SRC1, SRC proto-oncogene, non-receptor tyrosine kinase
Construction	The Human SRC (P12931-1) (Met 1-Leu 536) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Protein Purity	> 90 % as determined by SDS-PAGE
分子量	87.7 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Supplied as sterile 50 mM Tris, 100 mM NaCl, pH 8.0, 20% gly, 0.3 mM DTT.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Research Background	Proto-oncogene tyrosine-protein kinase SRC is a hydrophobic protein belonging to the SRC family kinase including nine members that is a family of non-receptor tyrosine kinases. SRC protein may exist in different forms: C-SRC and V-SRC. C-SRC is only activated under certain circumstances where it is required such as growth factor signaling, while V-SRC is constitutively active as opposed to normal SRC (C-SRC). Thus, V-SRC is an instructive example of an oncogene protein kinase whereas C-SRC is a proto-oncogene protein kinase. Inhibition of SRC with NR2A tyrosine phosphorylation mediated by PSD-95 may contribute to the lithium-induced downregulation of NMDA receptor function and provide neuroprotection against excitotoxicity.

参考文献

1. Juan Ma. et al., 2003, Neuroscience Letters. 348 (3): 185-189. 2. Czernilofsky AP. et al., 1980, Nature. 287: 198-203. 3. Beischlag TV. et al., 2002, Molecular and cellular biology. 22 (12): 4319-33.

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Src Protein, Human, Recombinant (His & GST)

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