TGFBR1 Protein, Human, Recombinant (mFc & Avi)

transforming growth factor beta receptor 1 (TGFBR1), a key stimulator of tumor proliferation and metastasis, was a direct target of miR‑98‑5p. miR‑98‑5p overexpression resulted in the downregulation of TGFBR1 and the suppression of the viability, proliferation, migration and invasion of A549 and H1299 cells. TGFBR1 Protein, Human, Recombinant (mFc & Avi) is expressed in HEK293 mammalian cells with C-mFc-Avi tag. The predicted molecular weight is 38.2 kDa and the accession number is P36897-1.

TGFBR1 Protein, Human, Recombinant (mFc & Avi)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 144,500
500 μg	約5 days	￥ 694,500
1 mg	約5 days	￥ 1,267,000

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生物学的情報

Human TGFBR1, mFc Tag captured on CM5 Chip via Protein A can bind Human Mature TGF beta 1, No Tag with an affinity constant of 0.35 μM as determined in SPR assay.

説明

Species	Human
Expression Host	HEK293 Cells
Tag	C-mFc-Avi
Accession Number	P36897-1
別名	tbetaR-I, TGFβRI, TGFbetaRI, LDS2A, ALK-5, TGF-beta RI, tβR-I, TGF-β RI, TGFR-1, TGFB1R1, ACVRLK4, LDS1A, AAT5, TGFBR1, TGF-bRI, SKR4
Construction	Leu34-Glu125
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	38.2 kDa (predicted). Due to glycosylation, the protein migrates to 50-55 kDa based on Tris-Bis PAGE result.

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	transforming growth factor beta receptor 1 (TGFBR1), a key stimulator of tumor proliferation and metastasis, was a direct target of miR‑98‑5p. miR‑98‑5p overexpression resulted in the downregulation of TGFBR1 and the suppression of the viability, proliferation, migration and invasion of A549 and H1299 cells.

参考文献

1. Jiang F, et al. MicroRNA-98-5p inhibits proliferation and metastasis in non-small cell lung cancer by targeting TGFBR Int J Oncol. 2019 Jan;54(1):128-138. doi: 10.3892/ijo.2018.4610. Epub 2018 Oct 29. PMID: 30387848; PMCID: PMC6255066.

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TGFBR1 Protein, Human, Recombinant (mFc & Avi)

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