Thioredoxin/TRX Protein, Human, Recombinant

Thioredoxin/TRX Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 11.7 kDa and the accession number is P10599.

Thioredoxin/TRX Protein, Human, Recombinant

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 138,000
500 μg	約5 days	￥ 447,500

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説明	Thioredoxin/TRX Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 11.7 kDa and the accession number is P10599.

Species	Human
Expression Host	E. coli
Tag	Tag Free
Accession Number	P10599
別名	thioredoxin, TRDX, DKFZp686B1993, MGC61975, TXN, TRX1, RP11-427L11.1, TRX
Construction	A DNA sequence encoding the C-terminal segment of human TXN (P10599) (Met 1-Val 105) was expressed and purified.
Protein Purity	> 97 % as determined by SDS-PAGE
分子量	11.7 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from sterile PBS, pH 7.5. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Thioredoxin, also known as ATL-derived factor, Surface-associated sulphydryl protein, SASP and TXN, is a nucleus, cytoplasm and secreted protein that belongs to the thioredoxin family. Thioredoxins are proteins that act as antioxidants by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. Thioredoxins are found in nearly all known organisms and are essential for life in mammals. Thioredoxin / TXN participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Thioredoxin / TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Thioredoxin / TXN nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Thioredoxin / TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

参考文献

1. Holmgren A , et al.,1989, J Biol Chem 264 (24): 13963-6. 2. Nordberg J, et al., 2001, Free Radic Biol Med31 (11): 1287-312. 3. Mustacich D, et al., 2000, Biochem J 346 (Pt 1): 1-8. 4. Mitchell D.A., et al., 2005, Nat. Chem. Biol. 1:154-158.

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Thioredoxin/TRX Protein, Human, Recombinant

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