Thioredoxin/TRX Protein, Mouse, Recombinant

Thioredoxin/TRX Protein, Mouse, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 11.7 kDa and the accession number is P10639.

Thioredoxin/TRX Protein, Mouse, Recombinant

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 88,500
200 μg	約5 days	￥ 151,500
500 μg	約5 days	￥ 307,500

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生物学的情報	Measured by its ability to catalyze the reduction of insulin. The reaction leads to precipitation, which can be measured by absorbance at 650 nm. The specific activity is >4 A650/min/mg.
説明	Thioredoxin/TRX Protein, Mouse, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 11.7 kDa and the accession number is P10639.

Species	Mouse
Expression Host	E. coli
Tag	Tag Free
Accession Number	P10639
別名	Txn, Txn1, thioredoxin
Construction	The Mouse Txn (P10639) (Met1-Val105) was expressed and purified.
Protein Purity	> 95 % as determined by SDS-PAGE
分子量	11.7 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Thioredoxin, also known as ATL-derived factor, Surface-associated sulphydryl protein, SASP and TXN, is a nucleus, cytoplasm and secreted protein that belongs to the thioredoxin family. Thioredoxins are proteins that act as antioxidants by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. Thioredoxins are found in nearly all known organisms and are essential for life in mammals. Thioredoxin / TXN participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Thioredoxin / TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Thioredoxin / TXN nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Thioredoxin / TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

参考文献

1. Holmgren A , et al.,1989, J Biol Chem 264 (24): 13963-6. 2. Nordberg J, et al., 2001, Free Radic Biol Med31 (11): 1287-312. 3. Mustacich D, et al., 2000, Biochem J 346 (Pt 1): 1-8. 4. Mitchell D.A., et al., 2005, Nat. Chem. Biol. 1:154-158.

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Thioredoxin/TRX Protein, Mouse, Recombinant

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