TLE3 Protein, Human, Recombinant (aa 484-772, GST)

The association between high TLE3 expression and a favorable response to taxane-containing chemotherapy regimens was validated in patients with non-serous ovarian cancer. That TLE3 expression may serve as a marker of chemosensitivity in taxane-treated patients with non-serous histologies. Transducin-like enhancer of Split3 (TLE3) serves as a transcriptional corepressor during cell differentiation and shows multiple roles in different kinds of cancers. TLE3 repressed CRC proliferation partly through inhibition of MAPK and AKT signaling pathways, suggesting the possibility of TLE3 as a biomarker for CRC prognosis.

TLE3 Protein, Human, Recombinant (aa 484-772, GST)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 161,000

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説明

Species	Human
Expression Host	E. coli
Tag	GST
Accession Number	Q04726-1
別名	transducin-like enhancer of split 3, GRG3, ESG3, HsT18976, ESG
Construction	The N-terminal fragment of Human TLE3 (Q04726-1) (Ser 484-Tyr 772) was fused with the GST tag at the N-terminus.
Protein Purity	> 78 % as determined by SDS-PAGE
分子量	58.5 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 0.15M NaCl, 5 mM GSH, pH 7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	The association between high TLE3 expression and a favorable response to taxane-containing chemotherapy regimens was validated in patients with non-serous ovarian cancer. That TLE3 expression may serve as a marker of chemosensitivity in taxane-treated patients with non-serous histologies. Transducin-like enhancer of Split3 (TLE3) serves as a transcriptional corepressor during cell differentiation and shows multiple roles in different kinds of cancers. TLE3 repressed CRC proliferation partly through inhibition of MAPK and AKT signaling pathways, suggesting the possibility of TLE3 as a biomarker for CRC prognosis.

参考文献

1. Villanueva CJ,et al.(2011) TLE3 is a dual-function transcriptional coregulator of adipogenesis. Cell Metab. 13(4): 413-27. 2. Carvalho LR,et al.(2010) Corepressors TLE1 and TLE3 interact with HESX1 and PROP1. Mol Endocrinol. 24(4): 754-65. 3. Nakaya HI,et al.(2007) Splice variants of TLE family genes and up-regulation of a TLE3 isoform in prostate tumors. Biochem Biophys Res Commun. 364(4): 918-23. 4. Brinkmeier ML,et al.(2003) TCF and Groucho-related genes influence pituitary growth and development. Mol Endocrinol. 17: 2152-2161. 5. Dasen JS,et al.(2001) Temporal regulation of a paired-like homeodomain repressor/TLE corepressor complex and a related activator is required for pituitary organogenesis. Genes Dev. 15: 3193-3207. 6. Douglas KR,et al.(2001) Identification of members of the Wnt signaling pathway in the embryonic pituitary gland. Mamm Genome. 12: 843-851. 7. Chen G,et al.(2000) Groucho/TLE family proteins and transcriptional repression. Gene. 249: 1-16.

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TLE3 Protein, Human, Recombinant (aa 484-772, GST)

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