TNF alpha Protein, Human, Recombinant (His & Avi)

TNF alpha Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 20.3 kDa and the accession number is P01375.

TNF alpha Protein, Human, Recombinant (His & Avi)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 80,000
500 μg	約5 days	￥ 321,500
1 mg	約5 days	￥ 535,000

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説明	TNF alpha Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 20.3 kDa and the accession number is P01375.

Species	Human
Expression Host	HEK293 Cells
Tag	C-His-Avi
Accession Number	P01375
別名	TNFα, APC1 protein, TNFSF2, TNF, TNF α, TNF-alpha, TNF-α, TNFSF1A, TNFATNF, TNFalpha, Cachectin, DIF, TNFA
Construction	Val77-Leu233
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	20.3 kDa (predicted). Due to glycosylation, the protein migrates to 21-25 kDa based on Tris-Bis PAGE result.

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism.Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

参考文献

1. Danese S, et al. Review article: the role of anti-TNF in the management of ulcerative colitis - past, present and future[J]. Alimentary Pharmacology & Therapeutics, 2013, 37(9):855-866.

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TNF alpha Protein, Human, Recombinant (His & Avi)

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