TRIB3 Protein, Human, Recombinant (GST)

カタログ番号 TMPY-04461

別名: SKIP3, C20orf97, tribbles pseudokinase 3, TRB3, SINK, NIPK

Tribbles homolog 3, also known as Neuronal cell death-inducible putative kinase, p65-interacting inhibitor of NF-kappa-B, SINK and TRIB3, is a Nucleus protein that belongs to the protein kinase superfamily and CAMK Ser/Thr protein kinase family and Tribbles subfamily. Highest expression Of TRIB3 is in liver, pancreas, peripheral blood leukocytes and bone marrow. It is also highly expressed in a number of primary lung, colon and breast tumors. TRIB3 is expressed in spleen, thymus, and prostate and is undetectable in other examined tissues, including testis, ovary, small intestine, colon, leukocyte, heart, brain, placenta, lung, skeletal muscle, and kidney. TRIB3 disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. TRIB3 may bind directly to and mask the 'Thr-38' phosphorylation site in AKT1. It binds to ATF4 and inhibits its transcriptional activation activity. TRIB3 interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. It interacts with MAPK kinases and regulates activation of MAP kinases. It may play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. TRIB3 does not display kinase activity.

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TRIB3 Protein, Human, Recombinant (GST)

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 91,500

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生物学的特性に関する説明

Technical Params

Product Properties

参考文献

説明

Species	Human
Expression Host	Baculovirus Insect Cells
Tag	GST
Accession Number	Q96RU7
別名	SKIP3, C20orf97, tribbles pseudokinase 3, TRB3, SINK, NIPK
Construction	The Human TRIB3 (NP_066981.2) (Met 1-Gly 358) was fused with the GST tag at N-terminus.
Protein Purity	> 90 % as determined by SDS-PAGE
分子量	65.8 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Supplied as sterile 50 mM Tris, 100 mM NaCl, pH 8.0, 0.5 mM Reduced Glutathione, 10% gly, 0.5 mM PMSF.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Research Background	Tribbles homolog 3, also known as Neuronal cell death-inducible putative kinase, p65-interacting inhibitor of NF-kappa-B, SINK and TRIB3, is a Nucleus protein that belongs to the protein kinase superfamily and CAMK Ser/Thr protein kinase family and Tribbles subfamily. Highest expression Of TRIB3 is in liver, pancreas, peripheral blood leukocytes and bone marrow. It is also highly expressed in a number of primary lung, colon and breast tumors. TRIB3 is expressed in spleen, thymus, and prostate and is undetectable in other examined tissues, including testis, ovary, small intestine, colon, leukocyte, heart, brain, placenta, lung, skeletal muscle, and kidney. TRIB3 disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. TRIB3 may bind directly to and mask the 'Thr-38' phosphorylation site in AKT1. It binds to ATF4 and inhibits its transcriptional activation activity. TRIB3 interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. It interacts with MAPK kinases and regulates activation of MAP kinases. It may play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. TRIB3 does not display kinase activity.

参考文献

1. Wu M. et al., 2003, J. Biol. Chem. 278: 27072-9. 2. Kiss-Toth E. et al., 2004, J. Biol. Chem. 279: 42703-8. 3. Ord D., et al.,2005, Biochem. Biophys. Res. Commun. 330: 210-8. 4. Schwarzer R. et al., 2006, Cell. Signal. 18:899-909.Greenman C. et al., 2007, Nature. 446:153-8.

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Keywords

TRIB3 Protein, Human, Recombinant (GST) SKIP 3 SKIP-3 SKIP3 C20orf97 tribbles pseudokinase 3 TRB3 SINK TRB 3 TRB-3 NIPK recombinant recombinant-proteins proteins protein