uPAR/PLAUR Protein, Mouse, Recombinant (His)

uPAR/PLAUR Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 31.4 kDa and the accession number is P35456-1.

uPAR/PLAUR Protein, Mouse, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
50 μg	在庫あり	￥ 58,500
100 μg	約5 days	￥ 100,000
200 μg	約5 days	￥ 171,000
500 μg	約5 days	￥ 346,500

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生物学的情報	Measured by its ability to bind with uPA-His in a functional ELISA.
説明	uPAR/PLAUR Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 31.4 kDa and the accession number is P35456-1.

Species	Mouse
Expression Host	HEK293 Cells
Tag	His
Accession Number	P35456-1
別名	uPAR, Cd87, u-PAR, plasminogen activator, urokinase receptor
Construction	The extracellular domain of Mouse PLAUR (NP_035243.1) precursor (Met 1-Thr 297) was expressed, fused with a polyhistidine tag at the C-terminus.
Protein Purity	≥ 97 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC.
分子量	31.4 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Urokinase plasminogen activator (uPA) and/or its receptor (uPAR) are essential for metastasis, and overexpression of these molecules is strongly correlated with poor prognosis in a variety of malignant tumours. uPAR and uPA levels in both resected tumor tissue and plasma are of independent prognostic significance for patient survival in several types of human cancer. This system has classically been thought to drive tumor progression by mediating directed extracellular proteolysis on the surface of migrating or invading cells, and intervening with this proteolysis by targeting uPAR has been proposed to represent a novel approach for inhibiting tumor progression. uPAR, also known as PLAUR or CD87, has been implicated in the growth, metastasis, and angiogenesis of several solid and hemotologic malignancies. uPAR is a highly glycosylated, 55-60kDa integral membrane protein linked to the plasma membrane by a glycosylphosphatidylinositol (GPI) anchor. It is part of a cell surface system that also consists of the serine protease uPA and several specific inhibitors (plasminogen activator inhibitors 1 and 2). Additionally, the analysis of CD87 (urokinase-type plasminogen activator receptor - uPAR) expression has a potential role in the diagnostic or prognostic work-up of several hematological malignancies, particularly acute leukemia and multiple myeloma.

参考文献

1. Romer J,et al.(2004) The urokinase receptor as a potential target in cancer therapy. Curr Pharm Des. 10(19): 2359-76. 2. Bn MC,et al.(2004) CD87 (urokinase-type plasminogen activator receptor), function and pathology in hematological disorders: a review. Leukemia. 18(3): 394-400. 3. Pillay V,et al.(2007) The urokinase plasminogen activator receptor as a gene therapy target for cancer. Trends Biotechnol. 25(1): 33-9. 4. Mazar AP. (2008) Urokinase plasminogen activator receptor choreographs multiple ligand interactions: implications for tumor progression and therapy. Clin Cancer Res. 14(18): 5649-55.

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uPAR/PLAUR Protein, Mouse, Recombinant (His)

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