VEGFR3/FLT4 Protein, Human, Recombinant (aa 25-776, His & Avi), Biotinylated

Vascular endothelial growth factor (VEGF) and its receptors VEGF-R1, -R2 and -R3 play important roles in tumor angiogenesis and are associated with poor prognosis in several solid tumors.VEGF-R1, -R2 and -R3 were highly expressed in CRC cells and stromal vessels. VEGF-R1 strong positive staining correlated with shorter survival after CRC surgery. VEGFR3/FLT4 Protein, Human, Recombinant (aa 25-776, His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 87.4 kDa and the accession number is P35916-1.

VEGFR3/FLT4 Protein, Human, Recombinant (aa 25-776, His & Avi), Biotinylated

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 187,000
500 μg	約5 days	￥ 746,000
1 mg	約5 days	￥ 1,248,500

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説明

Species	Human
Expression Host	HEK293 Cells
Tag	C-His-Avi
Accession Number	P35916-1
別名	PCL, FLT-41, LMPH1A, FLT-4, VEGFR3, PCLFLT41, VEGFR-3
Construction	Tyr25-Ile776
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	87.4 kDa (predicted). Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result.

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Vascular endothelial growth factor (VEGF) and its receptors VEGF-R1, -R2 and -R3 play important roles in tumor angiogenesis and are associated with poor prognosis in several solid tumors.VEGF-R1, -R2 and -R3 were highly expressed in CRC cells and stromal vessels. VEGF-R1 strong positive staining correlated with shorter survival after CRC surgery.

参考文献

1. Okita NT, et al. Vascular endothelial growth factor receptor expression as a prognostic marker for survival in colorectal cancer. Jpn J Clin Oncol. 2009 Sep;39(9):595-600. doi: 10.1093/jjco/hyp066. Epub 2009 Jun 17. PMID: 19535387.

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VEGFR3/FLT4 Protein, Human, Recombinant (aa 25-776, His & Avi), Biotinylated

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