Powder: -20°C for 3 years | In solvent: -80°C for 1 year
BW-A 78U is a PDE4 inhibitor (IC50: 3 μM). It is not inhibition on the lipopolysaccharide (LPS)-induced TNF-α release.
説明 | BW-A 78U is a PDE4 inhibitor (IC50: 3 μM). It is not inhibition on the lipopolysaccharide (LPS)-induced TNF-α release. |
ターゲット&IC50 | PDE4:3 μM |
細胞研究 | Mononuclear cells were incubated for 30 min with BW-A 78U (0.01-10 μM). The cells were then stimulated with lipopolysaccharide (10 μg/mL) overnight at 37°C in an atmosphere of 5% CO2 at 100% humidity. Cell-free supernatants were collected, centrifuged (2000 g), and stored frozen at -20°C before TNF-α determination. TNF-α concentrations in cell culture supernatants were determined by specific ELISA using a commercial kit. The sensitivity of the assay was 1 pg/mL. The absorbance at 450 nm was assessed with an ELISA reader. |
別名 | BW-A78U |
分子量 | 257.27 |
分子式 | C13H12FN5 |
CAS No. | 101155-02-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 45 mg/mL (174.91 mM)
You can also refer to dose conversion for different animals. 詳細
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BW-A 78U 101155-02-6 Metabolism PDE inhibit Inhibitor BW A 78U BW-A78U Phosphodiesterase (PDE) BWA 78U inhibitor