Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Importazole is an inhibitor of the nuclear transport receptor importin-β.
説明 | Importazole is an inhibitor of the nuclear transport receptor importin-β. |
In vitro | Importazole is an effective inhibitor of the Ran/importin-β interaction in vitro. Importazole specifically blocks importin-β-mediated nuclear import both in Xenopus egg extracts and cultured cells, without disrupting transportin-mediated nuclear import or CRM1-mediated nuclear export. When added during mitosis, importazole impairs the release of an importin-β cargo FRET probe and causes both predicted and novel defects in spindle assembly[1]. It also inhibits proliferation and induces apoptosis of multiple myeloma cells by blocking the NF-κB signal pathway in vitro[2]. |
細胞研究 | For all import and export experiments, HEK 293 cells stably expressing NFAT-GFP are grown on glass coverslips to approximately 50% confluency prior to drug treatment. In all cases, importazole is used at 40 μM and leptomycin B is used at 10 ng/ml. For controls, DMSO is used at a concentration of 0.4%. Ionomycin is added at 1.25 μM. Importazole and leptomycin B treatments are all for 1 hour. In all experiments cells are fixed with 4% formaldehyde prior to fluorescence microscopy. DNA is visualized with 1 μg/ml Hoechst dye. For quantification, 100 cells from each condition are analyzed and the percentage that shows nuclear accumulation of NFAT-GFP calculated.(Only for Reference) |
分子量 | 318.42 |
分子式 | C20H22N4 |
CAS No. | 662163-81-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 28 mg/mL(87.9 mM)
DMSO: 12.5 mg/mL (39.26 mM)
You can also refer to dose conversion for different animals. 詳細
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Importazole 662163-81-7 Apoptosis Others Inhibitor inhibit inhibitor