keep away from moisture
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Pepstatin (Pepsin Inhibitor S 735A) is a specific aspartic proteases inhibitor produced by actinomycetes, and inhibits the aspartic proteases cathepsin D, pepsin and renin.
パッケージサイズ | 在庫状況 | 単価(税別) | |||
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5 mg | 在庫あり | ¥ 7,500 | |||
10 mg | 在庫あり | ¥ 11,000 | |||
25 mg | 在庫あり | ¥ 22,500 | |||
50 mg | 在庫あり | ¥ 38,000 | |||
100 mg | 在庫あり | ¥ 63,000 | |||
200 mg | 在庫あり | ¥ 91,500 | |||
1 mL * 10 mM (in DMSO) | 在庫あり | ¥ 11,000 |
説明 | Pepstatin (Pepsin Inhibitor S 735A) is a specific aspartic proteases inhibitor produced by actinomycetes, and inhibits the aspartic proteases cathepsin D, pepsin and renin. |
ターゲット&IC50 | Hemoglobin-proctase:6.2 nM, Hemoglobin-acid protease:260 nM, Hemoglobin-pepsin:4.5 nM, Casein-pepsin:150 nM, Casein-acid protease:520 nM, Casein-proctase:290 nM |
In vitro | Pepstatin is a specific acid protease inhibitor with IC50s of 4.5 nM, 6.2 nM, 150 nM, 290 nM, 520 nM and 260 nM for hemoglobin-pepsin, hemoglobin-proctase, casein-pepsin, casein-proctase, casein-acid protease, and hemoglobin-acid protease, respectively [1]. Pepstatin inhibits the recombinant HIV protease (IC50: 250 μM). Pepstatin shows no effect on cellular protein synthesis and probably does not exert severe cell toxicity [2]. |
In vivo | Pepstatin has very low toxicity, with LD50s of 1090 mg/kg, 875 mg/kg, 820 mg/kg and 450 mg/kg for mice, rats, rabbits, and dogs by i.p. route, and > 2000 mg/kg for all species by the oral route. Pepstatin (0.5-50 mg/kg, p.o.) suppresses stomach ulceration of the pylorus in ligated Shay rats [1]. The bacterial motility in the pepstatin-treated gastric juice was measured at the pH values of 2.0, 3.0, 4.0, 4.5, and 5.0 (n = 5 for each pH). The bacteria remained motile significantly longer than in the in vivo experiments without pepstatin [4]. |
細胞研究 | Pepstatin A is freshly dissolved in DMSO at 7 mM. It is very slowly diluted (1:100) into the medium of HIV-infected H9 suspension cultures so that no pepstatin A precipitated (final concentration, 70 μM pepstatin A and 1% DMSO), and the cultures are incubated without change of culture medium for 48 hr. As a control, uninfected H9 cells are also incubated with pepstatin and in addition HIV infected and uninfected cells are incubated with 1% DMSO but without pepstatin [2]. |
動物実験 | To investigate the effect of pepsins on bacterial motility, similar experiments were performed, but the pepsin in the stomach was inactivated by rinsing the stomach with pepstatin (100 μl of a 2-mg/ml stock solution). Samples were taken and analyzed for bacterial motility at the test pH values of 2.0, 3.0, 4.0, 4.5, and 5.0 and at the same periods after application of the bacterial suspension as in the experiments with active pepsins [4]. |
別名 | Pepstatin A, Pepsin Inhibitor S 735A, Ahpatinin C |
分子量 | 685.89 |
分子式 | C34H63N5O9 |
CAS No. | 26305-03-3 |
keep away from moisture
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 1 mg/mL (1.46 mM)
H2O: Insoluble
DMSO: 25 mg/mL
You can also refer to dose conversion for different animals. 詳細
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
Pepstatin 26305-03-3 Autophagy Metabolism Microbiology/Virology Proteases/Proteasome Ubiquitination Proteasome HIV Protease Amino Acids and Derivatives Inhibitor inhibit Pepstatin A Pepsin Inhibitor S 735A Ahpatinin C inhibitor