Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Rotenone (Rotocide) is a natural product and a botanical insecticide. Rotenone is a mitochondrial electron transport complex I inhibitor that promotes the production of reactive oxygen species in mitochondria and induces apoptosis.
パッケージサイズ | 在庫状況 | 単価(税別) | |||
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サンプルについてお問い合わせ | |||||
500 mg | 在庫あり | ¥ 10,000 | |||
1 g | 在庫あり | ¥ 11,500 | |||
5 g | 在庫あり | ¥ 20,500 | |||
1 mL * 10 mM (in DMSO) | 在庫あり | ¥ 10,500 |
説明 | Rotenone (Rotocide) is a natural product and a botanical insecticide. Rotenone is a mitochondrial electron transport complex I inhibitor that promotes the production of reactive oxygen species in mitochondria and induces apoptosis. |
In vitro |
METHODS: Human leukemia cells HL-60 were treated with Rotenone (0-1000 nM) for 30 min and oxygen consumption was measured using a Clark oxygen electrode. RESULTS: Rotenone dose-dependently inhibited HL-60 cell respiration. Rotenone inhibited cellular respiration by more than 96% at 500 nM. [1] METHODS: 10th DIV cells were treated with Rotenone (20 nM) for 48 h. LDH levels were measured by LDH release assay. RESULTS: LDH release can be used as an indicator of general cytotoxicity. rotenone induced an increase in LDH release, and LDH activity increased more than 6-fold compared to the control group. LDH activity increased more than 6-fold compared to the control group.[2] |
In vivo |
METHODS: To establish a reproducible mouse model of Rotenone-induced Parkinson's disease (PD), Rotenone (2.5 mg/kg) was administered intraperitoneally to C57Bl/6 mice once daily for two weeks. RESULTS: Systemic exposure of mice to Rotenone resulted in progressive accumulation and regional spread of p129 aggregates, which preceded the maximal loss of DAn. [3] METHODS: To study neurotoxicity, Rotenone (30-100 mg/kg) was administered orally to C57BL/6 mice once daily for fifty-six days. RESULTS: The survival of Rotenone-treated mice at 30 mg/kg was unchanged from 28-56 days, although the survival of Rotenone-treated mice was reduced to approximately 70% within one week. 100 mg/kg Rotenone-treated mice showed a sudden decrease in survival after 28 days, and ultimately to approximately 15% after 56 days. A regimen of Rotenone given chronically at 30 mg/kg for 56 days is more useful for understanding the mechanisms of DA neurodegeneration. [4] |
植物由来 |
別名 | Rotenon, Paraderil, Barbasco, Rotocide, Dactinol |
分子量 | 394.42 |
分子式 | C23H22O6 |
CAS No. | 83-79-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
5% DMSO+95% Saline: 1.97 mg/mL (4.99 mM, precipitation)
DMSO: 18.33 mg/mL (46.48 mM), Sonication is recommended.
You can also refer to dose conversion for different animals. 詳細
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Rotenone 83-79-4 Apoptosis Autophagy Metabolism Dehydrogenase p53 Mitochondrial Metabolism Rotenon Paraderil Inhibitor Barbasco Rotocide inhibit Dactinol inhibitor