CD40 Ligand Trimer Protein, Human, Recombinant (hFc)

CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.

CD40 Ligand Trimer Protein, Human, Recombinant (hFc)

パッケージサイズ	在庫状況	単価(税別)
100 μg	お問い合わせ	￥ 96,000
500 μg	お問い合わせ	￥ 385,500
1 mg	お問い合わせ	￥ 642,500

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説明

Species	Human
Expression Host	HEK293 Cells
Tag	N-hFc
Accession Number	P29965
別名	CD40 Ligand, CD40LIGM, IGM, TNFSF5, TRAP, HIGM1, CD154, TNFSF5IMD3, CD40L, T-BAM, CD40LG
Construction	Met113-Leu261
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	74.9 kDa (predicted). Due to glycosylation, the protein migrates to 75-80 kDa based on Tris-Bis PAGE result.

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.

参考文献

1. Bjorck P , Filbert E , Zhang Y , et al. The CD40 agonistic monoclonal antibody APX005M has potent immune stimulatory capabilities[J]. Journal for Immunotherapy of Cancer, 2015, 3(S2):P198. 2. Pound, J. D. Minimal cross-linking and epitope requirements for CD40-dependent suppression of apoptosis contrast with those for promotion of the cell cycle and homotypic adhesions in human B cells[J]. International Immunology, 1999, 11(1):11-20.

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CD40 Ligand Trimer Protein, Human, Recombinant (hFc)

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