GSK3B Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 49 kDa and the accession number is Q9WV60.
説明 | GSK3B Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 49 kDa and the accession number is Q9WV60. |
Species | Mouse |
Expression Host | Baculovirus Insect Cells |
Tag | His |
Accession Number | Q9WV60 |
別名 | GSK-3, 7330414F15Rik, C86142, glycogen synthase kinase 3 β, 8430431H08Rik, GSK-3beta, GSK-3β, glycogen synthase kinase 3 beta, GSK3 |
Construction | The Mouse GSK3B (Q9WV60) (Met 1-Thr 420) was fused with a polyhistidine tag at the N-terminus. |
Protein Purity | > 75 % as determined by SDS-PAGE |
分子量 | 49 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Supplied as sterile 20 mM Tris, 500 mM NaCl, 25% glycerol, 0.2 mM DTT, pH 7.4. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. |
Research Background | GSK3B is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It Contains 1 protein kinase domain, and is expressed in the testis, thymus, prostate, and ovary and weakly expressed in the lung, brain, and kidney. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in the GSK3B gene have been implicated in modifying the risk of Parkinson's disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer's disease. GSK3B participates in the Wnt signaling pathway. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB, and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. GSK3B also plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. GSK3B phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which are critical for its role in bulge stem cell migration and skin wound repair. It may be required for early embryo development and neuron differentiation. |
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Please read the User Guide of Recombinant Proteins for more specific information.
GSK3B Protein, Mouse, Recombinant (His) GSK-3 7330414F15Rik C86142 glycogen synthase kinase 3 β C 86142 C-86142 8430431H08Rik GSK-3beta GSK 3 GSK-3β glycogen synthase kinase 3 beta GSK3 recombinant recombinant-proteins proteins protein