IL-6 Protein, Human, Recombinant

IL-6 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 20.95 kDa and the accession number is P05231.

IL-6 Protein, Human, Recombinant

パッケージサイズ	在庫状況	単価(税別)
20 μg	在庫あり	￥ 41,000
100 μg	約5 days	￥ 102,500
200 μg	約5 days	￥ 160,500
500 μg	約5 days	￥ 447,500

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生物学的情報

1. Immobilized Human IL-6 at 2 μg/mL (100 μL/well) can bind Human IL-6R hFc, the EC50 of Human IL-6R hFc is 3.0-10.0 ng/mL. 2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.1-0.8 ng/mL. 3.Loaded Recombinant Human IL-6R Protein, His Tag on His1K Biosensor, can bind Recombinant Human IL-6 Protein, no Tag with an affinity constant of 0.165 µM as determined in BLI assay (Routinely tested).

説明

IL-6 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 20.95 kDa and the accession number is P05231.

Species	Human
Expression Host	E. coli
Tag	Tag Free
Accession Number	P05231
別名	BSF2, HSF, IL-6, IFNB2, interleukin 6, HGF, Interleukin-6
Construction	A DNA sequence encoding the mature form of human IL6 (NP_000591.1) (Val30-Met212) was expressed with an initial Met at the N-terminus.
Protein Purity	≥ 95 % as determined by SDS-PAGE
分子量	20.95 kDa (predicted)

Endotoxin	< 10 EU per mg of the protein.
Formulation	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as a phosphorylated and variably glycosylated molecule. It exerts actions through its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined as an essential role in directing the transition from innate to acquired immunity.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

参考文献

1. Heinrich PC. et al. (2003). Principles of interleukin-6-type cytokine signalling and its regulation. Biochem J. 374: 1-20. 2. Rose-John S, et al. (2007) The IL-6/sIL-6R complex as a novel target for therapeutic approaches. Expert Opin Ther Targets. 11(5): 613-24. 3. Dinh W, et al. (2009) Elevated plasma levels of TNF-alpha and interleukin-6 in patients with diastolic dysfunction and glucose metabolism disorders. Cardiovasc Diabetol. 8:58.

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IL-6 Protein, Human, Recombinant

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