MAPKAPK3 Protein, Human, Recombinant (GST)

カタログ番号 TMPY-04378

別名: mitogen-activated protein kinase-activated protein kinase 3, 3PK, MAPKAP3, MAPKAPK-3, MAPKAP-K3, MK-3

The MAPKAP kinases are a group of MAP kinase substrates that are themselves kinases. In response to activation, the MAP kinases phosphorylate downstream components on a consensus Pro-X-Ser/Thr-Pro motif. Several kinases that contain this motif have been identified and serve as substrates for the ERK and p38 MAP kinases. Mitogen-activated protein (MAP) kinase-activated protein kinase 3, also known as MAPKAPK-3 and 3pK, is a member of the Ser/Thr protein kinase family. It is widely expressed in human tissues, with a higher expression level observed in the heart and skeletal muscle. No expression in the brain. MAPKAPK-3 is unique since it was shown to be activated by three members of the MAPK family, namely extracellular-signal-regulated kinase (ERK), p38, and Jun-N-terminal kinase (JNK). It is highly activated both by mitogens and by stress-inducing agents or proinflammatory cytokines and translocates to the cytoplasm from the nucleus. MAPKAPK-3 is exclusively activated via the classical MAPK cascade, while stress-induced activation of MAPKAPK-3 is mainly mediated by p38, however, the mechanism defining the specificity remains unknown.

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MAPKAPK3 Protein, Human, Recombinant (GST)

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 114,500

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生物学的特性に関する説明

Technical Params

Product Properties

参考文献

説明

Species	Human
Expression Host	Baculovirus Insect Cells
Tag	N-GST
Accession Number	A0A024R2W7
別名	mitogen-activated protein kinase-activated protein kinase 3, 3PK, MAPKAP3, MAPKAPK-3, MAPKAP-K3, MK-3
Construction	The full length of Human MAPKAPK3 (NP_004626.1) (Met 1-Gln 382)
Protein Purity	> 90 % as determined by SDS-PAGE
分子量	69 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Supplied as sterile 50 mM Tris, 100 mM NaCl, pH 7.5, 0.25 mM DTT, 0.1 mM EDTA, 0.5 mM PMSF, 10% glycerol.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Research Background	The MAPKAP kinases are a group of MAP kinase substrates that are themselves kinases. In response to activation, the MAP kinases phosphorylate downstream components on a consensus Pro-X-Ser/Thr-Pro motif. Several kinases that contain this motif have been identified and serve as substrates for the ERK and p38 MAP kinases. Mitogen-activated protein (MAP) kinase-activated protein kinase 3, also known as MAPKAPK-3 and 3pK, is a member of the Ser/Thr protein kinase family. It is widely expressed in human tissues, with a higher expression level observed in the heart and skeletal muscle. No expression in the brain. MAPKAPK-3 is unique since it was shown to be activated by three members of the MAPK family, namely extracellular-signal-regulated kinase (ERK), p38, and Jun-N-terminal kinase (JNK). It is highly activated both by mitogens and by stress-inducing agents or proinflammatory cytokines and translocates to the cytoplasm from the nucleus. MAPKAPK-3 is exclusively activated via the classical MAPK cascade, while stress-induced activation of MAPKAPK-3 is mainly mediated by p38, however, the mechanism defining the specificity remains unknown.

参考文献

1. McLaughlin, M.M. et al., 1996, J. Biol. Chem. 271:8488-8492. 2. Tanoue, T. et al., 2001, EMBO J. 20: 466-479. 3. Neufeld, B. et al., 2000, J. Biol. Chem. 275: 20239-20242. 4. Zakowski,V. et al., 2004, Exp Cell Res. 299: 101-109. 5. Voncken, J. W. et al., 2005, J. Biol. Chem. 280: 5178-5187.

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Keywords

MAPKAPK3 Protein, Human, Recombinant (GST) mitogen-activated protein kinase-activated protein kinase 3 MAPKAPK3 3PK MAPKAP3 MAPKAPK-3 MAPKAP-K3 MAPKAP-3 MAPKAP 3 MK3 MAPKAPK 3 MK-3 MK 3 recombinant recombinant-proteins proteins protein